Interleukin17Acts In Synergy With B Cell Activating Factor To Influence B Cell Biology In Pirmary Sjogren’s Syndrome

Objective To identify interleukin17and B cell–activating factor to influence B cellbiology by detecting the expression of BAFF in serum and labial salivary glands frompSS patients and the apoptosis rates of B cell cultured with Th17cell whichIL-17-siRNA、BAFF-siRNA Transfcted．Methods A total of40patients with pSS was referred to the department ofRheumatology and Immunology at Anhui Provincial Hospital from June2011to June2012. The expression of BAFF on salivary gland and serum from pSS patients andhealthy controls were detected by ELISA and Immunohistochemistry (22patients withpSS). BAFF and IL-17interfered with amplified Th17cells,and co-cultured with Bcell, flow cytometry was used to detect B cell’s apoptosis,immunoblot was used todetect supernatant antibody in5patients with pSS.Independent samples t test forstatistical analysis.Results1.In all pSS specimens, infiltrating inflammatory cells expressed BAFF,as didsome ductal cells,but acinar cells were negative.There were no expression of BAFF inthe controls. BAFF-positive cells numbers in the labial salivary glands of pSS patientswith focal infiltrating lymphocytes were more than that with non-focal infiltratinglymphocytes（888.3±371.8vs163.5±160.7, P<0.05）.and the percentage ofBAFF-positive lymphocytes cells over the total infiltrating lymphocytes in the salivary glands of pSS patients with focal infiltrating lymphocytes was higher than that withnon-focal infiltrating lymphocytes (P<0.05).2.The level of soluble BAFF in patientswith pSS was significantly higher than the controls(P<0.05).3.BAFF or IL-17transfection group, B cell apoptosis rate was significantly higher than non-transfectedgroup(P <0.05), and there was no significant difference compared with a separatecultured B cells (P>0.05).Conclusion BAFF may be involved in the process of local inflammatory damage ofthe pSS, it and IL-17may have a synergistic effect in abnormal B cell function.