Effect Of Intestinal Barrier Dysfunction And Bacteria Translation On Peripheral Blood CD4~+T Cells In AIDS Patients

Objective:To invastigate the effect of intestinal barrier dysfunction and bacterial translocation on peripheral blood CD4+T cells in AIDS patients.Methods:AIDS patients were classified into3groups based on their peripheral CD4+T cell counts:Group A:CD4+T cells<200/uL; Group B:200/uL<CD4+T cell <350/uL;Group C:CD4+T cells≥350cells/uL.ELISA measure3intestinal barrier injury biomarkers:serum diamine oxidase (DAO), intestinal fatty acid binding protein (iFABP) and D-lactate (D-LA), and3bacterial translocation biomarkers:endotoxin (LPS), anti-endotoxin core antibody (EndoCab) and soluble CD14(sCD14). We compared the levels of those biomarkers among patients in the different CD4+T groups, and subsequently studied the quantitative association among the intestinal barrier dysfunction and bacterial translocation biomarkers with peripheral CD4+T cell counts.Results:No significant deferences were observed between the3CD4+T groups on any of the6intestinal barrier dysfunction and bacterial translocation biomarkers. DAO and iFABP showed rise and fall trends, respectively, in groups with CD4+T cell counts changing from low to high, but the changes were not statistically significant. The levels of all3intestinal barrier injury biomarkers were very significantly correlated with the3bacterial translocation biomarkers.Conclusion:1) There are no differences on intestinal barrier damage and bacterial translocation in groups defined by CD4+T cell counts. The severity of intestinal barrier damage and bacterial translocation do not significantly associated with the change of CD4+T cell counts. The immune function of AIDs patients cannot be determined solely by the number of CD4+T cells in peripheral blood.2) The severity of intestinal barrier injury and bacterial translocation are positively correlated. Bacterial translocation increases as intestinal barrier injury becomes more severe.