The Research On The Relationship Of EBNA1, EBNA2, SS56,ACA And Sjogren’s Syndrome

Background Sjogren’s syndrome (Sjogren’s syndrome, SS) is a chronic autoimmune disease involving lacrimal gland, salivary gland and other exocrine glands. It characterized by a large number of lymphocytic infiltration. About half of the SS patients may have system damage, the most serious of which can progress to lymphoma, Common lymphomas are B-cell lymphoma and non-Hodgkin’s disease (NHL). The etiology and pathogenesis of SS is not clear, the cause may be related to genetic factors, environmental factors such as viral infections, sex hormones. Researchers generally believed that the viral infection was not the direct causative factors of the disease, it may be a motivating factor. The majority study of domestic and foreign scholars confirmed that EBV is closely related to the pathogenesis of SS. Plenty of evidence related EBV to the occurrence of lymphoma. EBNA1and EBNA2are core antigen which express in EBV latency. Researchs indicate that EBNA1/EBNA2antibody≤1associated with the risk of lymphoma. This article aims to study the relationship between EBNA1,EBNA2and SS. SS56is a new type of protein found in2001, its structure is related to52KDRo/SSA antigen, it may be involved in the pathogenesis of SS. Anticardiolipin antibodies (ACA) is a kind of antiphospholipid antibodies, It significance in SS remains uncertain.This article also studied the relationship between SS56,ACAand SS.Objective1. To explore the significance of EBNA1and EBNA2protein with SS.2. To explore the significance of SS56antibody,ACAin the pathogenesis and diagnose of SS.Methods1. Collected SS follow-up cases data from January1,2004to2012, October30in our hospital. Collected the wax block of labial gland and serum of SS patients and recorded cases in general, clinical features, laboratory examination.The grouping criteria:According to diagnostic criteria for classification of Europe and the United States meeting in2002,the patient was divide into pSS (primary Sjogren’s syndrome) group and the sSS (secondary Sjogren’s syndrome) group.2. The expression of EBNA1and EBNA2in labial gland of32patients with pSS,7cases of sSS patients and4normal was detected with Envision im-munohistochemical method (light yellow to yellow-brown granular material app-ear in the cytoplasm, nucleus and coloring higher than the background color is represent positive). Integrated optical density was measured and quantitative an alysis was carried on.Come to a conclusionby use statistical methods.3. The expression of EBNA1-IgG antibody and SS56antibody in the serum detected by ELISA.4. The expression of ACA in the serum detected by chemiluminescence.Results:1.EBNA1and EBNA2expression in the labial gland:(1) EBNA1and EBNA2expressed positively in the plasma cells, B lymphocytes in the labial gland. A small amount of EBNA2expressed positively in ductal epithelial cells (DEC), and acinar epithelial cells (AEC), no EBNA1and EBNA2expressed in the normal control group.(2) EBNA1-positive rate of SS labial gland was71.8%(28/39), EBNA2-positive rate was69.2%(27/39),The positive rate of EBNA1and EBNA2had no difference (p>0.05), There is no EBNA1The EBNA2expression in normal control group.The positive rates of EBNA1and EBNA2in pSS group were68.8%(22/32),65.6%(21/32), The both positive rates of EBNA1and EBNA2in sSS group were71.4%(5/7). There was no significant difference in the EBNA1-positive rate in pSS group and sSS group (p>0.05), There was no significant difference in the EBNA2-positive rate in pSS group and sSS group (p>0.05).(3)27of39SS labial gland co-expressed EBNA1and EBNA2.1case express ed EBNA1only.(4)The patients with Integrated optical density of EBNA1/EBNA2>1(17cases) and Integrated optical density of EBNA1/EBNA2=1(10cases) appeared to have significant no difference in clinical manifestations (dry mouth, dry eyes, mouth ulcers, vision loss, fever, fatigue, joint pain, mumps, enlarged spleen),Age, course of the disease and laboratory tests (WBC, RBC, PLT, HB, W-ESR, AP, GP, ALT, AST, IgG, IgA, C3, C4, etc.).2. There was no difference in the positive rate of EBNAl-IgG antibody in the serum of pSS group, the sSS groups and the normal control group,(p>0.05).3. The positive rate of SS56antibody in serum pSS patient (84.4%) was higher than the normal control group (43.5%)(p<0.05)4. There was no significantly different in the positive rate of ACA in the in the serum of pSS (6.3%) and the control group (4.5%)(p>0.05)Conclusion:1. The EBNA1-positive rate of labial gland in SS patients was71.8%, EBNA2-positive rate of SS labial gland was69.2%, expressed higher than normal labial gland (p<0.05), EBV may be involved in the pathogenesis of SS.2. The positive rate of EBNA1-IgG antibodies had no difference in the serum of SS patients and normal control group,(p>0.05).3. The positive rate of SS56antibody in serum of14SS cases was85.7%, higher than the SSA (78.5%), SSB (35.7%) and normal control group, SS56antibody was one of the serous family members in the SS.It could be used as a diagnosis of serous indexes in SS patient.4. The positive rate of ACA antibodies had no significant difference in the serum of pSS patients and normal control group,(p>0.05)...