Research Of The Molecular Mechanism Of Polyphylloside Against K562Leukeamia Cell

[Objective] To explore the inhibiton and apoptosis of K562Leukeamia Cell induced by Polyphylloside and its molecular mechanisms.[Methods](1):The Polyphylloside treatment of K562cells and the establishment of the experimental and control groups(2):The cells were collected, extracted total RNA.The search for human beta-catenin gene and beta-actin mRNA in the full-length sequence of primers and were designed extracted by Trizol total RNA, reverse transcriptase.The SYBR method of real-time PCR detection beta-catenin gene mRNA levels relative to express differences. Each sample three times a parallel experiment was repeated.(3):The cells were collected,extracted total protein. The protein concentration was determined using the BCA method, and carried SDS-PAGE, transferred to a membrane, anti-beta-catenin antibody incubation, the secondary antibody, ECL chromogenic, tabletting exposure photos record the results, the analysis of people beta-catenin protein levels relative expression of different.Each sample three times a parallel experiment was repeated.[Results](1) Respectively0.625μ.g/ml,1.25μg/ml,2.5μg/mland5μg/ml concentration of Polyphylloside processing human leukemia cells K562cell lines24h,beta-catenin mRNA with respect to the relative expression level of the control group were0.88±0.081(P0.203),0.65±0.106(P0.051),0.57±0.064(P0.047),0.41±0.056(P0.042),2.5μg/ml,5μg/ml dose difference between statistically significant (P <0.05); the histogram visually demonstrated Polyphylloside inverse relationship between beta-catenin inhibition dose;(2) Respectively0.625μg/ml,1.25μg/ml,2.5μg/mland5μg/ml concentration of Polyphylloside processing human leukemia cells K562cell lines24h,beta-catenin protein with respect to the relative expression level of the control group were0.47±0.075(P0.810),0.38±0.020(P0.318),0.32±0.070(P0.042),0.27±0.006(P0.031),2.5μg/ml,5μg/ml dose difference between a significant difference (P<0.05), beta-catenin in1.25μg/ml dose group relative to the0.625μg/ml dose group had not been affected but increased,The histogram showed beta-catenin inhibition dose was in addition tol.25μg/ml dose group outside Polyphylloside inverse relationship.[Conclusion] Polyphylloside can inhibit the growth of K562Leukeamia Cell. Polyphylloside can induce apoptosis of human Leukeamia Progenitor cell. The Polyphylloside may inhibit the Wnt signaling pathway by inhibiting the expression of beta-catenin mRNA and protein levels in K562cells, thereby inhibiting the proliferation of leukemic cellsand growth, and promote apoptosis.