Research Of Polyphylloside Against Human Leukeamia Progenitor Cell

AIM:To explore the inhibiton and apoptosis of Human Leukeamia Progenitor cell induced by Polyphylloside and its mechanisms.METHODS:(1):20cases of myeloid leukemia (AML, CML) patients and20cases of lymphocytic leukemia (ALL CLL) patients were recruited, Separation out the bone marrow of the human Leukeamia progenitor cell by using Ficoll, And detected leukemia progenitor cells content by Flow Cytometry (FCM)(2):Cell proliferation was tested by MTT with different time and its results were expressed as IC50values.(3):Cell Apoptosis was observed by dual dyeing of AnnexinV-FITC&PI.RESULTS:(1) Polyphylloside could inhibit the growth of Myeloid human Leukeamia Progenitor, and inhibition of proliferation was concentration-time dependence (2) Polyphylloside could inhibit the growth of Lymphocytic human Leukeamia Progenitor, and inhibition of proliferation was concentration-time dependence, Compared with Cyclophosphamide group, the inhibition level of Polyphylloside was higher than Cyclophosphamide.(3) Polyphylloside could induce apoptosis of Myeloid human Leukeamia Progenitor, and the apoptosis level of Polyphylloside was higher than Epirubicin group.CONCLUSION:Polyphylloside can inhibit the growth of human Leukeamia Progenitor cell. Polyphylloside can induce apoptosis of human Leukeamia Progenitor cell. The inhibiton effect of Polyphylloside on human Leukeamia Progenitor cell may through the way of apoptosis induction.