Effects And Mechanism Of Qi Chinese Medicine Combined With Itopride Of The Rate Of Gastric Emptying In Diabetic Rats

Objective:Diabetes is a clinical common diseases and frequently-occurring disease, its complications incidence rate of22%, involving the digestive tract lead to delayed gastric emptying, known as diabetic gastroparesis. Mchugh and Erbas statistical., In patients with type1or type2diabetes at least more than50%associated with diabetic gastroparesis, mainly neuropathy, Cajal interstitial cells (ICC) lesions gastrointestinal hormone, hyperglycemia, microvascular disease, pyloric pylori (Helicobacten pylori, HP) infection and other factors. This issue through ALX-induced diabetic rat model, choice of Qi Chinese medicine with itopride combination therapy observed the therapeutic effect of the different groups of diabetic gastroparesis which is confirmed that combination therapy compared with monotherapy advantage, and in the neuromuscular lesion level to elucidate the possible mechanism of its occurrence, then lay the foundation for further clinical studies.Methods:The first stage:the purchase of animals (SPF SD male rats were randomly selected from70, weight220±20g) reagents, experimental preparation.The second stage:The diabetic rat model.Seventy rats, were randomly divided into five groups after observing one weeks.Group A:control group(n=10),Group B:diabetes group(n=15), Group C:Qi Chinese Medicine group (n=15), Group D:Itopride group (n=15) and Group E:Itopride unifies Qi Chinese Medicine group (n=15). Fasted for24hours before modeling, B, C, D, E group rats by intraperitoneal injection of5%aqueous solution of alloxan120mg/kg once a day for two consecutive days. After72hours, for three consecutive days fasting blood glucose more than13.6mmol/1for molding success. Phase III:packet feeding. A, B group given normal diet and normal saline daily2ml/200g the C group fed daily on the basis of given normal feed Chinese medicine2ml/100g, D group on the basis of given normal feed fed daily in Iraq the care will benefit12.5mg/100g, E group on the basis of given ordinary feed daily gavage the itopride12.5mg/100g+TCM2ml/100g. Observed blood glucose, body weight, food intake of rats in each group.Phase Ⅳ:the animals were killed, specimen processing. After feeding24weeks later, all rats were forbidden to eat in 24hours,and then After that, all rats were absolutely non-dieted24hours again, and were killed60minutes later after pouring0.1%methyl orange according to0.2ml/only,then took out the stomaches, observed the residue of methyl orange., Calculation of gastric emptying rate (the emptying rate=standard value-residual value). Gastric tissue specimens were detected for the morphological changes of the rat gastric myenteric plexus and smooth muscleResults:(1)Compared with normal group, diabetic rats stomach methyl orange residue more, gastric emptying time was significantly prolonged(P<0.01).(2)Given the Qi the medicine or itopride group treatment can improve gastric emptying rate of diabetic rats (P<0.05); Qi medicine joint itopride group to improve the effect of diabetic rat gastric emptying was significantly better than with Qi medicine group or itopride group (P<0.01).(3)Normal group of gastric smooth muscle cells arranged rules closely moderate gap between the cells, no vacuoles changed appearance change. Model group gastric smooth muscle, smooth muscle cell cytoplasm is pale, translucent, vacuolar degeneration. Western medicine group gastric smooth muscle, smooth muscle cell cytoplasm is pale, translucent, vacuolar degeneration, or even collapse. Chinese Medicines gastric smooth muscle, smooth muscle cells arranged still rules, vacuolar degeneration. Chinese medicine western medicine group gastric smooth muscle, smooth muscle cells arranged in more regular, small vacuoles.Conclusions:1、 Qi medicine joint itopride can effectively promote gastric motility in diabetic rats with qi Chinese medicine or itopride, group was better than in promoting gastric emptying (P<0.01).2Alloxan-induced DM rats delayed gastric emptying, gastric smooth muscle morphological changes of the stomach myenteric plexus decrease in the number of the nNOS staining positive neurons was significantly reduced, suggesting that diabetic gastroparesis with the the nerve cells in the smooth muscle and myenteric there are inextricably linked the relationship. the Application of Qi Chinese medicine and Itopride can be improved to varying degrees emptying condition, and the joint-user effect is more obvious(P<0.01), and its mechanism may be the protective effect of the Smooth muscle and the nerve plexus in the smooth muscle and myenteric, to reduce its injury, thus for the clinical diabetic Gastroparesis Treated with provides a new therapeutic approach.