| The Berberis Amurensis belongs to the Berberis Linn of the Berberidaceae. Berberis Amurensis,alias Barberry,dog breast root,with the heat-clearing and damp-drying,purging fire and detoxification,anti-inflammatory and acesodyne efficacy;Tropism of taste:bitter,cold;mainly treats dysentery,upper respiratory tract infection,acute conjunctivitis,mastitis,boils and pyogenic intections,enteritis,chronic hepatitis embolism.Berberis Amurensis is a the Korean Folk common drug.In addition, Recent studies have found that the Berberis genus are generally rich in alkaloids and other compounds,But the system of the chemical constituents and pharmacological activities of Berberis property of their respective plants have not complete.The research of pharmacological activity and constituents of the Berberis Amurensis very few,This experiment is make further screening and appraise the anti-tumor active site of the Berberis Amurensis on the basis of the preliminary study of the water extract and alcohol extract of the Berberis Amurensis has obvious anti-tumor activity,And make a systematic study of the chemical composition of the Berberis Amurensis.and to seek and find the monomer commpounds with anti-tumor activity, and to provide a basis for the further development of new drug treatment of malignant tumors.The experiment set up the ultrasonic extraction and heat reflux extraction method to obtain the ethanol extract.Ultrasonic extract was diluted with water,and with petroleum ether,methylene chloride,ethyl acetate,n-butanol extraction.The heate reflux extraction after with the acid and alkali treatment,and with petroleum ether,methylene chloride,ethyl acetate,n-butanol extraction.Set up S180sarcoma method,screening effective fraction about resisting tumor in the Berberis Amurensis is the ethyl acetate extract.Silicagel column chromatography and other modern chromatographic method to separate and purify the Berberis Amurensis extract and elucidate1H-NMR,13C-NMR, MS and other modern pop and physical and chemical methods.Thirteen compounds were separated and identified from it.They are the berberine (1),jatrorrhizine (2), epiberberine (3),palmic acid (4),myristic acid (5),Pentadecanoic acid (6),campesterol (7),palmatine (8),β-sitosterol (9),stigmasterol (10),nonanoic acid (11),stigmast-4-en- 3-one (12),columbamine (13).The MTT Experiments show that the Compound3,8and13have a toxic effect on HepG-2.The IC50with the Compound8is89.91μg/mL.According to the report that the compounds1,2,8have a good anti-tumor activity.Compounds3-13are first isolated from the Berberis Amurensis. |
