The Association Of CYP2A6Polymorphism With Susceptibility Of Chronic Obstructive Pulmonary Disease

Chronic Obstructive Pulmonary Disease (Chronic obstructive pulmonary disease) is a serious respiratory disease, now widely recognized that the individual genetic susceptibility factors and environmental factors predisposing factors that cause chronic obstructive pulmonary disease. In recent years, with the continuous development of molecular biology, environmental and genetic factors in the occurrence and development of COPD in affect each other, the relationship is very complex. Smoking so far been recognized as COPD, the most important predisposing environmental factors, this study focuses on the cytochrome CYP2A6gene polymorphisms and susceptibility to chronic obstructive pulmonary disease (COPD). Find related genes that cause the development of COPD, smoking environmental factors interaction analysis, to provide a theoretical basis for the COPD screening of high-risk groups, early prevention and treatment.This study were mainly collected from subjects peripheral blood2ml, saved at-80℃cryopreservation and genomic DNA was extracted. All patients had complete data exclude high blood pressure, coronary heart disease, diabetes, cancer and other systemic diseases with a genetic predisposition; to exclude bronchiectasis, bronchial asthma, interstitial lung disease, tuberculosis, chest trauma, surgery or malformations respiratory disease. Selected the same period in healthy subjects and pulmonary clinic acute upper respiratory tract infection as a control group. All patients with chronic obstructive pulmonary disease are in line with the Chinese Society of Respiratory Disease Branch of chronic obstructive pulmonary disease study group developed chronic obstructive pulmonary disease (COPD) treatment guidelines (2007Revision) of the diagnostic criteria. Synthetic CYP2A6primer sites genotypes tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), gel electrophoresis to determine the results. Smoking index, age, FEV1/FVC (first second forced expiratory volume/forced vital capacity), FEV1/PRE%(first second forced expiratory volume/) t-test; gender, smoking history line x2test. Two groups of genotypes and alleles line x2test their statistical significance Logistic regression was used to calculate the OR and95%confidence interval (CI), used to assess the risk of wild genotypes of the disease. The above calculation by SPSS11.0statistical software analysis, P<0.05was a statistically significant difference.The results show that patients with COPD CYP2A6locus gene deletion (del) and wild type (wt) genotype frequency was17.5%,82.5%, and the healthy control group were40.5%,59.5%, frequency of distribution of the two sets of genesThe difference was statistically significant (x2=5.000, P=0.025), carry CYP2A6wt genotype more carry CYP2A6del genotype suffering from COPD increased risk (OR=3.214,95%CI=1.128-9.156). Smokers carrying CYP2A6wt genotype compared carry CYP2A6del genotype suffering from COPD increased risk (OR=4.176,95%CI=1.11-15.67, P<0.05), and in non-smokers, carrying CYP2A6wt, CYP2A6del genotype COPD risk was no significant difference between suffering.Thus we have come to the CYP2A6gene polymorphism may be risk factors of COPD. While the wild-type (wt) may be a predisposing factor of smokers suffering from COPD.