Acinethbacterb Amannii Resistance Analysis And The Carbon Blue Enzyme Detection

Objective:Understand the hospital clinical separation of acinetobacter baumannii separation drug resistance rate of clinical distribution characteristics, dynamic observation of the resistance to change; Detect penicillium carbon alkene antibiotic resistance of acinetobacter baumannii(CRAB) carbon penicillium enzyme genes and investigate the related factors of infectio (Environment of cross infection, antibiotics selective pressure, etc) Provide the basis for clinical treatment and effective control of hospital infection.Methods:By using the method of retrospective analysis of2001-2011yearly check out of the1571strains of acinetobacter baumannii clinical distribution and drug resistancesituation of statistical analysis.Collection of40strains resistant to carbon penicillium alkene antibiotics and has multiple drug resistance of acinetobacter baumannii,Using the disc diffusion method (K-B method) for drug sensitivity test,A modified Hodge experiment carbon alkene enzyme phenotypic screening.Application of polymerase chain reaction (PCR) to detect penicillium carbon olefine enzyme genes. To have a close contact with the40patients were related to medical personnel hand environmental objects (equipment door handle operation table) to monitor the surface disinfection effect.Results:Acinetobacter baumannii mainly comes from the respiratory tract specimens, a total of800strains of, Account for50.9%;Followed by wound secretion specimens, a total of546strains, accounting for34.8%; Clinical distribution is given priority to with acute588strains, accounting for37.4%; Respiratory461strains, accounting for29.3%; Acinetobacter baumannii to in beta lactam type of quinolone、aminoglycoside、penicillium carbon alkene antibiotic resistant rate showed a trend of increased year by year. Of penicillium carbon alkene>40%, Other three kinds of drug are>80%.High sensitivity to Cefoperazone/Sulbactam.40strains of penicillium notatum carbon alkene antibiotic resistant acinetobacter baumannii(CRAB) modified Hodge test are positive. With40training result as penicillium carbon alkene drug resistance of acinetobacter baumannii patients in close contact medical personnel hand disinfection test results of10cfu/cm2.Try to ICU environment (equipment door handle operation table) disinfection test result is the total number of colonies5cfu/cm2.Respiratory and urinary surgery neurosurgery environment (equipment door handle operation table) disinfection test result is the total number of colonies10cfu/cm2.Conclutions:1)Coverage of acinetobacter baumannii to commonly used antimicrobial drug resistance situation is serious. The carbon alkene enzymes antibiotic resistant rate above40%. And the trend of rising year by year.2)Our carbon penicillium alkene drug-resistant acinetobacter baumannii is one of the main resistance mechanism to produce OXA-23type carbon alkene enzyme, not detected IMP、VIM model of metal enzyme and OXA-24carbon alkene enzyme.3) Preliminary investigation penicillium caused our carbon alkene resistant acinetobacter baumannii(CRAB) infection factors associated with the application of antibiotics may be caused by the selective pressures.