| Objective: Smurf1belong to the E3the ubiquitin-proteasome connection members of the family of enzymes. The ubiquitin-proteasome mark needs to be thedegradation of protein, then the marked protein in the protease body being degraded.Smurf1mainly through the ubiquitin-proteasome Smad1and to participate in the Smad5negative control BMP of signal transduction pathways. Smurfl in BMP induces boneplays in the process of negative regulatory role. This experiment for studying the mainpurpose in rat orthodontic tooth mobile process, Smurf1periodontal tissue in theexpression, this paper discusses the relationship between the teeth Smurf1and moving.Method:Select a8-week-old21, male, SD rats, weight is in180-220g. Randompoints, seven groups, each of them only for a set of three. In SD rats dental establishorthodontic tooth mobile model, to the left of maxillary teeth for experimental bow side,on the right side of maxillary teeth compared to bow. The experiment of a selectionspecial micro planting nail as orthodontics a fight to nickel titanium as near to the aboveprecision extension spring traction force value of about40g, pull the maxillary molarteeth to move in the first close. Based on the classic a month orthodontic return cycle,and set up1day group,3days group,5days group,7days group,14days group,21days group,28days group, according to the group, in the,3,5,7,14,21,28day1time ratheart perfusion put to death, the specimen, internal and external fixation. Take themaxillary molar rats on both sides and the surrounding tissue as a specimen preparationand slice, and HE conventional dyeing SABC and immunohistochemical method, testexperimental rat orthodontic tooth in the process of moving Smurf1periodontal tissuein the expression changes. By using the computer analysis software gray valuemeasurement, and the statistical analysis SPSS17.0analysis software. Results:In the experimental orthodontic tooth mobile process, Smurf1periodontaltissue in the expression and distribution as the change of time there strength of specificdistribution. The experimental results indicate that the Smurf1in tension side is higherthan the pressure side expression. Grey value analysis showed that, periodontal tissueSmurf1expression in the seventh day of the peak,28days basic close to normal controlgroup level.Conclusion:Smurf1periodontal tissue in the positive expression, Smurf1participate in rats that orthodontic tooth in the process of moving bone rebuilt. Smurf1as BMP (BMPs) cells inhibitory factor, and that accompanies BMPs expression, thisexperiment revealed periodontal tissue Smurf1in the expression and distribution, tipSmurf1in limiting BMPs oerexpressed process may play an important role. |
