Expressions And Significance Of Survivin And Bcl-2in Laterally Spreading Tuomrs

Objeetive:Laterally spreading tumo（rLST）is considered as a superficial colorectaltumor originated from the colorectal mucous, it was characterized by the tumors largerthan1cm in diameters, grow laterally rather than vertically. Therefore, the lesion formand develop of LST is certain special, it’s different from any other colorectal adenomas,it has higher potential risk associated with colorectal cancer rather than theprotruded-type colorectal adenoma.Current investigations aim is on clinical, endoscopicdiagnosis and endoscopic therapeutic aspects of LST, but it’s very weak onmorphological and cancerous way. Our study aim is to find out the morphological andclincopathological feathers. Analyze the protein expression of Survivin and Bcl-2inLST、PA and colorectal cancer tissue by detecting their expression in this study.Method:The experiment is divided into LST group, PA group, colorectal cancergroup and normal control group.25cases for LST,15for PA,15for colorectal cancerand15for normal, and then compare their pathologic features of LST and PA base onpatient’s age, location, size, tumor histology.Immunohistochemistry(IHC) was used todetect the protein expression of survivin and bcl-2in tissue. Using χ~2detect and analyzethe dynamic of survivin and bc1-2in four groups.Results:1、 The clinical and histopathological features:In age,gender,tumor histologybetween LST and PA was not found significant difference (P>0.05);The size of LST(23.4±17.32mm) was significantly larger than that of PA (4.67±1.54mm, P<0.001).LST more common in right hemicolon (60.0%),followed by the rectum (24.0%).However PA more common in left hemicolon(46.7%),followed in rectum(33.3%).Therefore, there was a significant difference to the location of the lesion between theLST and PA (P<0.05).2、IHC results: The positive staining of survivin protein were mainly located in the cytoplasm. The positive expression rates in LST, PA, colorectal cancer and normalmucosa were96.0%（24/25)、80%（12/15）、86.7%（13/15）、60%（9/15）. Therewas significant difference in expression of survivin between LST, PA, colorectal cancerand normal mucosa (P<0.05). There was no significant difference between LST and PAgroup (P>0.05). The positive staining of bcl-2protein is mainly located in the cytoplasm,part in the membrane. The positive expression rates in LST, PA, colorectal cancer andnormal mucosa were96.0%（24/25)、80%（12/15）、86.7%（13/15）、80%（12/15）.Therewas significant difference in expression of bcl-2between as well as between LST, PA,colorectal cancer and normal mucosa(P<0.05) but there was no significant differencebetween LST and PA group (P>0.05).3、The expression correlation of survivin and bcl-2: we didn’t find any relationshipbetween survivin and bcl-2in LST (rs=-0.28,P=0.175), and the similar result in PA(rs=-0.051，p=0.864) and colorectal cancer group(rs=0.357，p=0.192).Conclusions:1、LST is located in the right hemicolon frequently,has been greater than the PA.2、There was no significant difference between LST and PA group (P>0.05).Itproves both of them are closely related with colorectal cancer, both of them areprecancerous disease.3、Both of survivin and bcl-2may be early indicators of malignancy determination4、There was no expression correlation between survivin and bcl-2.