Different Vibration Frequency Stimulation Matrix Bone Marrow Stromal Cells In The Body Experiment Research Differentiation

Objective: To investigate the effect of the different low vibration frequency onOsteogenic differentiation of in vivo Bone marrow stromal cells in themicroenvironment of the vivo Bone defect, and to quantify the optimal low vibrationfrequency.Methods: Select only40New Zealand rabbits, The left key difference inapproach to establish the hou4*4mm of bone defect model, and implanted BMSCsand take off calcium bone complex, Randomly divided into5groups: group A0Hz,group B12.5Hz, C of25Hz, D group frequency50Hz, E group100Hz frequency,Each group are applied for0.3g acceleration of sine wave exciting, vibration30minutes every day for four weeks, Four weeks later, put to death animals, take humeralspecimens were observed in, electron, slice observation staining of alkaline phosphataseanalysis, RT-PCR method to detect Ⅰ type collagen protein (Pre-Col1a). Using thestatistical analysis software SPSS13.0.Results: General observation: in group A bone soft tissue defect adhesion,hematoma of machine, and gradually evolved into the soft texture of granulation tissue,without obvious callus formation; B, C, D, E group bone defect of the hematoma hascompletely machine, granulation tissue have started to turn bone cartilage process, bonedefect cavity wall place is with thin layer callus formation, irregular shape, colorgloomy, press have impedance, and cavity wall bone join closely not easy separation,One group C, D group bone defect in the whole callus uniform filling the cavity, thesurface model form is good, the height and weeks wall is flat, grey color, press thequalitative hard.By electron microscopy observation: group A bone defect no callus formation, A fibrous tissue defect filling bone; The B group, the group C, D, E group of bone defectwhich numerous bone cells and less matrix, osseous callus formation, callus fillingevenly. Group C, D group bone structure histologic healing generally high level, callusformation arrange compact bone cells in bone trabeculae tissue, bone trabeculaesurrounding bone mother cells are arranged rules.Alkaline phosphatase (alkaline phosphatase, ALP) dyeing: each group all showedthat different degree of positive dyeing, group A bone cells in the sample after dyeing ofpositive signal counting less, and bone cells positive show color shallow, group B, C, D,E group of group of the more positive signals, bone cells positive show color is deeper,A group of alkaline phosphatase after dyeing, bone trabeculae seen in A fibrous tissueinto, the more cartilage groups appear, but have been generated bone tissue. Group B, C,D, E group of group is in the trabecular bone gap both fibrous tissue zhang into, themore cartilage groups appear, note the amount of osteoblasts, bone defect and cut endsto form good osseous connection, model form is good.Ⅰ type collagen protein (Pre-Col1a) relative expression: in group A Ⅰ typecollagen protein (Pre-Col1a) expression was significantly lower than the group B, C, D,E group of group. Another group C, D group Ⅰ type collagen protein (Pre-Col1a)express relative amount above group D, E group.Conclusion:Results show that different low frequency vibration are able tostimulate the body in small environment of BMSCs osteoblast differentiation; Amongthem with25Hz, frequency50Hz frequency of the low frequency vibration is mostevident.