Multicentric Urothelial Cancer Clonal Origin Of Heterozygote Deficiency Research

Objective: Urothelial carcinoma is the most common urological malignant tumors,which have high incidence characteristics of multi center. At present about urothelialcarcinoma multicentricity, potentially malignant biological behavior and the mechanismis not clear. The study of multifocal urothelial carcinoma multiple tumor foci of4microsatellite loci of D9S171, IFNA, D9S177, TP53LOH type detection, elucidatingthe multicentric urothelial carcinoma of the clonal origin. Further understanding ofurothelial cancer etiology, occurrence and development mechanism and biologicalcharacteristics and process, its early diagnosis, treatment options, prognosis andpostoperative monitoring and provide theoretical basis for the development of genetherapy.Methods: Extraction from20patients with multicentric foci of urothelial cancerin patients with a total of43foci (all by our institute cancer pathology diagnosis wasurothelial carcinoma) and the corresponding normal tissue DNA, selecting9,17onchromosome polymorphism of4microsatellite markers of D9S171, IFNA, D9S177,TP53, respectively in20patients with multicentric urothelial cancer tissues andcorresponding normal urothelial tissue DNA was amplified by PCR. In complete PCRproduct of denaturing polyacrylamide gel after electrophoresis ethidium bromidestaining observed in the UV transmittance of light to be photographed and withLabworks3.0gel analysis software to analyze, the incidence of the statistical loss ofheterozygosity, and then judge its clonal origin.Results: In12cases (12/20) detected by the primary tumor tissue gene DNA andthe corresponding multicenter cancer tissue gene DNA in1sites with the same LOHtype; there were2(2/20) the primary tumor tissue gene NDA and the correspondingmulticenter cancer tissue gene DNA in1of the same microsatellite loci have the same type of LOH, at the same time multicenter foci in other new microsatellite loci detectedthe occurrence of LOH;1cases (1/20)(for the recurrent cases) the primary tumortissue gene DNA and the corresponding multicenter cancer tissue gene DNA in differentmicrosatellite loci occurred with LOH; in1cases (for recurrent cases)(1/20) detectedby the primary tumor and the corresponding concurrent carcinoma lesions with differenttypes of LOH;1cases (1/20) only multicentric foci detected the occurrence of LOH;20cases in3cases (3/20) in the4microsatellite polymorphism marker loci were notdetected in LOH.Conclusions:Clonal origin of the multicentric urothelial carcinoma monoclonalorigin-based, repeated recurrence of urothelial cancer presence of polyclonal origin, i.e.independent of possible.