Study On The Inhibitory Effect Of Resveratrol On Lymphoma Cells And Impact Of The Pro-apoptotic Protein Caspase-3

Study on the inhibitory effect of resveratrol on lymphoma cells and Impact of thepro-apoptotic protein caspase-3Objective:Because BJAB cells have the same source with the most of orbital lymphomacells,we choose BJAB cells(a human B cell lymphoma cells)as the research object tostudy the inhibitory effect of resveratrol on BJAB cells and Impact of thepro-apoptotic protein caspase-3.The results of the study can provide an experimentalbasis for Ocular adnexal malt lymphoma(Ocular adnexal mucosa-associated lymphoidtissue lymphoma).Methods:1、The BJAB cells are reanimated and cultured.Observe cell morphology andgrowth conditions in a high-powered microscope to understand the characteristics ofthe cells.2、The experiment was divided into resveratrol drug groups, doxorubicinpositive control group and blank control group. The concentration of resveratrol druggroups were25μmol/L,50μmol/L,100μmol/L and the concentration ofdoxorubicin positive control group was40μmol/L.Acting on BJAB cells respectivelyto observe morphological changes in24,48,72h. Different groups BJAB cellsproliferation inhibition rate were determined by CCK-8.Observing the proliferationinhibition on BJAB cells in groups of different concentrations of the drug at differenttimes.3、Detecting the protein expression of caspase-3by western blot and analysingthe impact of the BJAB cell apoptosis pathway in different concentrations of the drugat different times.Results:1、BJAB cells grew well and passed stably.The shape of cells were round orellipse and the caryons were round.2、 Different drug concentrations of resveratrol drug groups and doxorubicinpositive control group aiming at BJAB cells24h,48h,72h,can inhibit cell proliferationin varying degrees.CCK-8assay OD values were lower than the control group, andstatistical analysis, the gap was statistically significant (P <0.05).3、Western blot test results shows that, compared with the blank control group,different drug concentrations of resveratrol drug groups and doxorubicin positivecontrol group aiming at BJAB cells24h,48h,72h,caspase-3protein expression risingin varying degrees.Conclusion:1、Resveratrol within a certain concentration range can inhibit BJAB cellproliferation and different concentrations of resveratrol effect different at differenttimes. Concentration and time can affect cell proliferation, in which the time-dependent effectis obvious.When the concentrationof resveratrol drug group were100μmol/L,showing time-dependent.Although time-dependent were not exsit when the concentration ofresveratrol drug group were25μmol/L and50μmol/L,however,after72h,theconcentration of50μmol/L lower cell activity and cell death more, so theconcentration will affect cell activity, affect cell proliferation.2、Resveratrol within a certain concentration can inhibit cell proliferation byaffecting the expression of caspase-3protein at different times.The rising of caspase-3protein expression on different drug concentrations of resveratrol drug groups werenot obvious,but at72h, caspase-3protein expression was significantly elevated in adose-dependent.3、According to the results of this experiment, we concluded that resveratrol alsohas a certain role in the inhibition of cell proliferatio for Ocular adnexal maltlymphoma.Then the results can supply the experimental and theoretical basis for theexperimental study in the future.