Role Of Ferric Iron Transporters When Iron Accumulation In Brain After Rat Intracerebral Hemorrhage

Object:Iron accumulation after hemolysis in intracerebral hemorrhage(ICH) trigger several pathways to transport and metabolize this iron. There have been few studies on the fate of iron after ICH and on the spatio-temporal relationship between iron overloading and iron-handling proteins that may regulate iron. There are few data in iron handling pathways after ICH. Our study is to observe the dynamic expression of the ferric iron deposit, the transferrin (Tf)/transferrin receptor(TfR) and ceruloplasmin (CP) in different time after ICH and their spatio-temporal patterns.Methods:In this study, forty eight ten-week-old SD rats were randomely divided into two groups:sham operation groups, cerebral hemorrhage(ICH) groups.Sprague-Dawley rats received an infusion of CollagenaseⅣ into the right basal ganglia and were killed at1,3,7.14day. MALLORY’s method was used for ferric iron staining, and brain iron deposits was determined. Brain Ff/TfR/CP around hematoma were examined by immunohistochemistry ((IHC)).Results:ICH resulted in ferric iron overload in the brain. A marked increase in brain iron was not cleared within14days. The expression of Tf in all groups increased and TfR/CP at1day,3day and7day intensified in ICH compared with the operation controls, and they reached a plateau at3-7day in ICH models. Correlations test showed that iron sediment granules positively correlated with Tf/TfR (r=0.468-0.521.P<0.01); CP expression is paralleled with Tf and TfR (r=0.521-0.634, P<0.01).Conclusions:Iron excessive accumulation after ICH activate Tf/TfR/CP expression. Tf/TfR/CP may play a role in clearing away iron when iron overload in brain.