| Objective:To study the hypoglycemic effect of gastric bypass surgery on T2DM rats withdifferent gastric pouch size, and explore its possible therapeutic mechanisms.Methods:Male SD rats were fed on high sugar and high fat diet to establish the T2DM ratmodel. It was done by intraperitoneal injection of small doses streptozotocin to therats. The rats were then randomized into small gastric pouch group（group A）, largegastric pouch group (group B), and sham surgery group (group C). Body weight, foodintake, fasting plasma glucose (FPG), postprandial plasma glucose (0.5h-PPG),plasma insulin (INS), glucagon-like peptide-1(GLP-1) of the rats were monitoredbefore and after1week,4weeks,8weeks,16weeks of the surgery. The rats werekilled16weeks after surgery and the pancreas were collected and made pathologysection. Islet morphology and insulin immunohistochemical expression wereobserved.Results:1. The body weight of rats was not significantly different (P>0.05) in the firstweek after surgery. There was no significant difference of body weight (P>0.05)between group B and group C in the4th,8thand16thweeks. The body weight of groupA reduced significantly (P <0.05) compared with other groups in the same period.2. Food intake in1,4,8,16weeks after surgery: Food intake of group Adecreased significantly (P <0.05), compared with the other groups. There was nosignificant difference between group A and group C(P>0.05).3. FPG and0.5h–PPG in1,4,8,16weeks after surgery: There was nosignificant difference (P>0.05) between group A and group B in the same period. Theblood glucose decreased significantly (P <0.05) in group A and group B, comparedwith group C. 4. Fasting insulin in group C was significantly lower than other groups (P <0.05)in the16thweek.5. GLP-1in the16thweek: GLP-1in group A and group B were higher than thatin C.The difference was statistically significant (P <0.05). There was no significantdifference (P>0.05) of the GLP-1between group A and group B.6. Results of HE16weeks after surgery: Islet tissue in group A and Group Bhave larger number, more complete envelope and more cells than that of group C. Thestaining area of insulin under immunohistochemistry in Group A and group B is largerthan that of group C.Conclusion:1. Gastric bypass surgery effectively reduce blood glucose of T2DM rats in theshort term (16weeks). Gastric pouch size has no significant effect on hypoglycemiceffect of gastric bypass.2. Gastric bypass surgery with small gastric pouch has significant effect inweight loss on T2DM rats.3. Gastric bypass surgery improves the secretory function of the pancreatic isletsof T2DM rats. |
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