The Permeability And The Protein Adsorption Properties Of Titania Nanotubes Arrays Biofiltration Membrane

Kidney disease has become another major disease that threat human’s health. Atpresent there are more than500million people with different kinds kidney disease,millions of people died in related disease of heart head blood-vessel disease. Althoughkidney transplant is a good method to slove patients into urenia renal replacement therapy,but there is a great limit because the kidney donors are little. Now the replacement therapyof hemodialysis become a successful maintained replacement therapy method after thekidney lose funtion. Therefore, there is a great significance of the research on biologicaldilysis membrane for hemodialysis therapy.Highly ordered TiO₂nanotubes array can be get by anodization. After corrosion, theend is opened, and we can get a through-hole nanotubes array membrane. The membranecan be used for biological filtration and biological dialysis.The paper studies the preparing of highly ordered TiO₂nanotubes array membrane.Thenanograss can be cleaned by surface corrosion and a two-step anodizatiaon, we get TiO₂nanotubes array with clean surface. Different anodization potential get different diametersof TiO₂nanotubes array. We can get a flow through nanotubes array membrane by etchingthe bottom of the TiO₂nanotubes membrane that spalling from the Ti. We can get someconclusions by the experiment. Compared with surface corrosion method, better orderedand more stable TiO₂nanotubes array is get by a two-step anodizatiaon. Open-ended TiO₂nanotubes array can be get by etching the bottom of the TiO₂membrane using HF.The bestetching time is15min.The next research is about the diffusion characteristics of TiO₂nanotubes membrane,the static adsorption and dynamic adsorption of Bovine Serum Albumin （BSA） on TiO₂nanotubes membrane. Photoelectric Colorimeter and Uv Spectrophotometer arerespectively used to research the influence produced to the diffusion characteristics ofPhenol Red and BSA of different time and different nanotubes. The diffusioncharacteristics is connect with the diameter of nanotubes. Larger diameter, better diffusion characteristics. Phenol Red is better than BSA. We have investigated the adsorption ofBovine serum albumin （BSA） on TiO₂nanotube membranes both static adsorption anddynamic adsorption using Fluorescence spectrometer, FTIR and XPS measurements. Wefound that the amounts of protein adsorptions are related with membranes, time, thediameter of TiO₂nanotubes and the flow rate of BSA solution.The amounts of proteinadsorption is about8—9μg/mm²in static adsorption of all membranes. In dynamicadsorption the amounts of protein adsorption on the TiO₂nanotube membranes（6—7μg/mm²ï¼‰ is samaller than other membranes （7—8μg/mm²ï¼‰; the adsorption is largerwhen the flow is5.5ml/min than2.5ml/min and8ml/min.At last, the paper introduces the new design of a dialysis device chip. TiO₂Membrane,Regenerated Cellulose Fiber Membrane, Mixed Cellulose Fiber Membrane and PESMembrane are respectively used to dialysis the plasma contained vitamin B12, urea andphosphate by the dialysis device. Then calculate the clearence of different membranes.Study the influence of BSA to dialysis by BSA solution. The results show that the dialysisabilities of TiO₂Membrane is the best, Mixed Cellulose Fiber Membrane and PESMembrane are better than Regenerated Cellulose Fiber Membrane; the clearence ofvitamin B12is the smallest, the urea’s is the largest of all membranes except RegeneratedCellulose Fiber Membrane. Little protein adsorbed on membranes, most pores are open.Protein losing is less than16%, it gradually become balance. The whole dialysis process isnot affected by the adsorption of BSA. All of this confirmed that the chip can be used indynalysis, provided a basis to the research of portable artificial dialysers.