The Expression And Clinical Significance Of Cyclin B1and TGF-β1in Non-small Cell Lung Cancer

Objective：To investigate the expression of Cyclin B1and Transforminggrowth factor-beta1(TGF-β1) in non-small cell lung cancer(NSCLC) and theirrelationship to clinicopathological characteristics and prognosis of tumors.Methods：The expressions of Cyclin B1and TGF-β1in62cases of humanNSCLC tissues and30cases of normal lung tissues, who have integrity clinical data,were detected by2-step plus immunohistochemistry. The correlations between theexpressions of Cyclin B1and TGF-β1alone or the co-expression and theclinicopathologic features as well as theirs prognosis were statistically analyzed. Dataanalyses were performed using PASW Statistics18.0. The difference between groupswas evaluated by the Chi-square test for categorical data. Correlation was determinedby Spearman analysis. Survival rate was calculated using the Kaplan-Meier method.The difference in survival rate was analyzed by the log-rank test. Multivariateanalyses were performed by using COX proportional hazards modeling.The level ofsignificance was set at P<0.05.Results：The expression postive rate of Cyclin B1and TGF-β1in thecarcinoma tissues were46.77%and56.45%, respectively. They were much higherthan those in normal tissue sample (16.67%and10%, respectively). Cyclin B1expression was closely correlated with tumor diameter, differentiation and TNM stage(P<0.05), while TGF-β1expression was associated with lymph node metastasis andTNM stage (P<0.05). Kaplan-Meier analysis showed that the survival rate of NSCLCwas significantly affected by tumor diameter, differentiation, lymph node metastasis,TNM stage, and expression of Cyclin B1or TGF-β1(P<0.05).Multivariate COXmodel analysis revealed that differentiation, lymph node metastasis, TNM stage,expression of TGF-β1and were independent prognostic factors ofNSCLC(P<0.05),and combined expression of Cyclin B1and TGF-β1were independent prognostic factors of NSCLC either(P<0.05).The expression of Cyclin B1waspositively correlated with that of TGF-β1(P<0.05).Conclusion：The expression positive rate of Cyclin B1and TGF-β1in thecarcinoma tissues were much higher than those in normal tissue sample respectively.Cyclin B1expression was closely correlated with tumor diameter, differentiation andTNM stage, while TGF-β1expression was associated with lymph node metastasis andTNM stage. The expression of Cyclin B1was positively correlated with that ofTGF-β1. Differentiation, lymphatic metastasis,TNM stage, expression of TGF-β1,combined expression of Cyclin B1and TGF-β1are independent prognostic factors ofNSCLC. Our findings suggest that Cyclin B1and TGF-β1may act synergistically inthe occurrence, development, invasion and metastasis of NSCLC, both of which areup-regulated in NSCLC tissues. The co-expression of Cyclin B1and TGF-β1, whichis closely related to malignancy, may serve as a biomarker for predicting prognosis.