The Effects And Mechanisms Of The Rh-GH On Dilated CardiomyoPathy In The Rat

ObjectiveTo study the effect of rats recombinant growth hormone(rh-GH)on the morphology andfunction of left cardiac ventricle in rats with idiopathic dilated cardiomyopathy(DCM).MethodsGrowth hormone treatment group (group R, n=18) DCM model rat injectd0.15IU/kgrhGH daily, saline treatment group (group S, n=18) with normal salineintraperitonealinjection, normal control group Group N, n=20) does not take any measures.After12weeks of treatment, Doppler echocardiography Figure measured cardiac index, blood wasmeasured IGF-1, IGFBP-3, cTnI, cTnTand NT-proBNP, polygraph was measured hemodynamic parameters were killed observe the heart and liver, the histopathologicalchanges of the lung.Result1serum IGF-1, IGFBP-3in rh-GH treatment group rat was significantly higher than thesaline sham treatment group rat and the control group rat, NT-proBNP was significantreduce compare with the other two groups(P <0.05);2cTnI and cTnT was significant lower compare with the saline sham treatment grouprat and the control group rat(P <0.05);3LVDd、LVDs、IVS、LVPWT in rh-GH treatment group rat was significantly higherthan the saline sham treatment group rat, the difference was statistically significant (P<0.05), compared with the blank control group of rats was not statistically significant (P>0.05);FS and EF in rh-GH treatment group rat was higher than the saline sham treatmentgroup rat, the difference was statistically significant(P <0.05), compared with the blankcontrol group rats was not statistically significant(P>0.05);4CVP and LVEDP in rh-GH treatment group rat was lower than the saline shamtreatment group rat, the difference was statistically significant(P <0.05), LVP and HRcompared with saline sham treatment group increased, the difference was statisticallysignificant(P <0.05);5rh-GH treatment group rat’s liver and lung congestion, HE staining under lightmicroscope and electronic fiber mirror observation rh-GH irreversible myocardialpathological changes of the DCM, but can inhibit the development in the baddirection, picric acid-Sirius red staining polarizing microscope, rh-GH treatment ofmyocardial col I accounted for (38.95±1.95)%, col Ⅲ accounted for (32.52±3.38)%, among the three groups was statistically significant(P <0.05).Conclusions1ultrasonic testing results suggest that the small dose of rh-GH treatment for12weeks, can significantly increase myocardial systolic and diastolic heart failure inrats, improve heart function, reversing the adverse ventricular reconstruction, increasedmyocardial cell contraction component. 2Group R group serum cTnI and cTnT levels of Group S was significantlydecreased, suggesting that a small dose of rh-GH treatment can reduce the incidence ofmyocardial damage.3Group R rats plasma IGF-1and IGFBP-3levels of NT-proBNP level declineprompted a small dose of rh-GH treatment antagonistic GH/IGF-1axis disorders, blockingthe development of heart failure vicious cycle.4picric acid Sirius red staining polarizing microscope observations suggest that a smalldose of rh-GH intervention can reduce non-cardiac infarct zone collagen content, improvethe proportion of collagen type reverse collagen remodeling.5HE staining observed in liver tissue, lung tissue pathological changes andhemodynamic test results suggest that a small dose of rh-GH intervention can reduce thesystemic and pulmonary vascular resistance, improve liver, lung congestion, improveexercise tolerance.6HE staining and scanning electron microscopy results suggest that a small dose ofrh-GH intervention can inhibit myocardial cell hypertrophy and necrosis.