The Methylation And MRNA Expression And Clinical Significance Of DAPK, RARβ2Gene In Thyroid Nodular Disease

Objective To study the gene of deathassociated protein kinase, DAPK in Nodular thyroiddisease in patients peripheral blood, the CpG island state of gene promoter methylation ofretinoic acid receptor, RARβ2and mRNA expression and then discuss its clinicalsignificance: the thesis aims to look for new tumor markers and then provides the basis forresistance to nodules treatment and targeted therapy for thyroid nodule and tumor.MethodsWe collected170cases of clinical patient from First Affiliated Hospital of Baotou medicalcollege between2007.9-2011.12(including41cases of nodular goiter,32cases of thyroidadenoma，34case of Grave，s disease，40case of Thyroid inflammation in hashimotogroup，23cases of thyroid carcinoma and30healthy control subjects who were takenlimosis vein blood. The thease examines gene methylation state of DAPK and RAR betavia application of the method of specific Methylation-specific PCR，MSP. It alsoexamines gene expression of mRNA existing in peripheral blood DAPK, RARβ2viaapplication of the method of Reversed transcriptive polymerase chain reaction RT-PCR. Itanalyzes gene methylation of peripheral blood, RARβ2and the relationship between thegene expression DAPK by using the statistical software SPSS13.0.Results (1)compared with normal control group (P=0.000,0.000, P <0.05), gene has high rate ofmethylation （39.1%，43.5%） in the thyroid cancer DAPK, RAR beta2with a significantdifference;(2) compared with normal (P=0.033, P <0.05), DAPK methylation rate（21.8%） are high among the thyroid gland tumor group, which has a statisticalsignificance; Thyroid inflammation in hashimoto group RARβ2gene has high rate ofmethylation, compared with normal control group (P=0.001, P <0.05), and has thesignificant difference;(3) in the calcification point or lymph node enlargement of thethyroid cancer, DAPK, RARβ2gene has high rate of methylation (fisher value=0.040,0.018,0.029,0.019, P <0.05), a statistical significance, in the thyroid cancer group DAPK,RARβ2gene methylation and of the patient’s age, gender, nodules size comparison fishervalue>0.05, no statistical significance.(4) DAPK gene in the thyroid cancer, thyroidgland tumors mRNA expression or express reduce loss, compared with normal control group (P=0.000,0.003, P <0.05), significant difference; RARβ2gene in the thyroidcancer, thyroid inflammation in merger hashimoto nodules mRNA express reduce orexpress is missing, and normal control group (P=0.000, compared to0.002, P <0.05)have significant differences.(5) DAPK, RARβ2gene expression to reduce or lack andgene promoter area abnormal methylation involved; Gene methylation and mRNAexpression was negatively correlated.Conclusion (1) DAPK, RAR β2gene in the thyroidcancer in peripheral blood are high in methylation rate while the mRNA express is reducedor lost in expression; the lack DAPK gene in the thyroid gland tumors has high rate ofmethylation, methylation rates lower than thyroid cancer group, reduce mRNA expression;RARβ2gene in the thyroid inflammation in the peripheral blood is hashimoto highmethylation state, reduce mRNA expression.(2) In the thyroid cancer, gene methylation ofDAPK, RARβ2has no significant differences with gender, age, size of the nodule, but isrelevant to nodules calcification and neck lymph node enlargement.(3) Joint test RARβ2,DAPK gene methylation state can obviously increase sensitivity of thyroid cancer, whichmay become a thyroid cancer early diagnosis of effective index.(4) The loss of geneexpression in DAPK, RAR beta2is relevant to gene promoter area abnormal methylation.