The Material Basis Study On Anti-tumor Activity Of Dandelion And Licorice

Currently, the main clinical treatment methods of breast and cervicalcancer are chemotherapy, radiation and surgery. Surgical treatmentinfluences the normal life of the patient. Chemotherapy and radiation canplay a therapeutic role to some extent, but its resistance and side effectsoften lead to quit of treatment. The advantages of Chinese traditionalmedicine are low toxicity and multi targets, which can effectively avoidthe emergence of drug resistance. The establishment of a method on theunderstanding of activity of Chinese medicine will reflect its holistic andcomprehensive effect and provide better evaluation of the quality oftraditional Chinese medicine with theoretical and practical significance.Since most of chemical compounds into the body through metabolismwill eventually be discharged into urine and bile, this study is based on thetheory of direct role material basis. In this study, rat urine after oraladministration of dandelion and licorice extract is the research object,MCF-7and Hela cells are the obeserving objects. MTT assay and PI singlestaining was applied to evaluate the effect of dandelion and licoriceextracts on proliferation and cell cycle of human breast cancer MCF-7celland cervical cancer. Integrated application of a variety of modernseparation techniques, medicated urine and anti-breast cancer activity partwere analyzed to clarify the material basis of the dandelion and liquorice inthe body. The results of this research will be presented as follows:1. Medicated urine of dandelion and licorice extract was used as drugsource to evaluate the inhibitory activity on MCF-7breast cancer cells andHela cervical cancer cells by MTT assay. The study found that dandelion and licorice extracts inhibit the activity of tumor cell proliferation in vivo.The effects of medicated urine on cell cycle of MCF-7and Hela cells wereinvestigated by PI staining. The results showed that medicated urine ofdandelion extract arrested both MCF-7and Hela cells at the G2/M phase.The medicated urine of licorice extract increased the MCF-7cell rate of Sand G2/M phase, decreased the rate of G1phase.2. A rapid UPLC-Q-TOF-MS assay was developed to preliminaryexplore and identify absorbing components and metabolites afteradministration of dandelion and licorice extract. UPLC-Q-TOFelectrospray ionization was applied in positive and negative ion mode toqualitatively analyse the Chinese medicine extract, blank rat urine anddandelion and licorice medicated urine with methanol as extracting reagent.By comparing fingerprints of blank urine and medicated urine afteradministration, we analysed absorption components and metabolites ofdandelion and licorice extract in vivo with Metabolynx software.78masspeaks were detected in dandelion aqueous extract, while13mass peakswere detected in medicated urine of dandelion aqueous extract. We alsofound62mass peaks in licorice aqueous and methanol extract and27masspeaks in medicated urine of licorice extract. Besides, Metabolynx softwarewas used to find potential metabolites in data processing.3. Further screening of anti-tumor activity prototype components andmetabolites of dandelion water extract in vivo was carried out by usingmodern analytical techniques, such as high-performance liquidchromatography (HPLC), gas chromatography (GC) and massspectrometry. We evaluated anti-tumor activity of segmented urine byMTT assay and analysed segments urine which significantly inhibitedMCF-7cell proliferation by GC-MS. Segments5,6and12of dandelionmedicated urine was found to have the highest inhibition rate of MCF-7cells. By GC-MS analysis,22components and metabolites were found inthe three sections. Dandelion terpene alcohol and lupeol are most likelyactive substances of dandelion extract to inhibit proliferation of MCF-7 breast cancer cell, which provides basis for further screening of anti-tumoractivity substances.