The Dynamic Changes Of Tert During The Development Of SD Rats’ Hepatocellular Carcinoma Intervention From All Trans Retinoic Acid Or Selenium Combined With Oxymatrine

Objective: In this study, based on SD rat liver cancer model built in our lab, all trans retinoicacid（ATRA） or selenium（Se）, combining with Oxymatrine（OMT） respectively were used as intervention, toexplore the dynamic changes of telomerase reverse transcriptase（TERT） in hepatic tissues and some bodyindexes of the rat. This study will provide some references for studying the molecular mechanism ofhepatocarcinogenesis, and looking for a new liver cancer therapy.Methods: Diethylnitrosamine was used as carcinogen to establish the rat modles of liver cancer indifferent phases by intragastric administration, OMT+ARTA and OMT+Se were used as intervention drugsduring this process. liver tissues were taken in the2^nd、4^th、6^th、8^th、10^th、12^th、14^th、16^th、18^thweekrespectively. The contents of TERT were studied and determined by immunohistochemistry and Westernblot.Results:①According to the pathologic slices, the individuals in the treatment groups showed primaryinflammatory reaction in the2^ndto4^thweek, severe inflammatory reaction（SIR） in the6^thto8^thweek,adenoma hyperplasia（AH） in the10^thto12^thweek, appeared atypical adenoma hyperplasia（AAH） in the14^thto16^thweek, cholangiocarcinoma（CCC） in the16^thto18^thweek.②immunohistochemistrial results:Positive rate of TERT in induced group and intervention groups were significant higher than control groupwhich showed none positive result in the whole progress of hepatocarcinogenesis; Positive rate of TERT inOMT+ATRA group was significant lower than induced group during AH and AAH phases （P<0.05）;Positive rate of TERT in Se+ATRA group was significant lower than induced group during the AH phase（P<0.05）, and highly significant lower during the AAH phase （P<0.01）. Special staining of TERT wasmainly concentrated in the cytoplasm, and also can be found in the nucleus during the AAH or CCC phase.③W estern blot:The content of TERT in control group was little（almost none） during progress ofhepatocarcinogenesis; The content of TERT in the induced group gradually increased in the whole phasesof the hepatocarcinogenesis; The content of TERT in the intervention groups were lower than in theinduced group during the process of hepatocarcinogenesis, expcially in AH phase, were significant lowerthan the induced group （P<0.05）; At the AAH phase, The content of TERT in OMT+Se group was significant lower than the induced group （P<0.05）, however there was no statistical difference betweenOMT+ATRA group and the induced group.Conclusions: The expression of TERT gradually increased during the process ofhepatocarcinogenesis induced by DENA. The intervention of OMT+ARTA and OMT+Se can decrease theexpression of TERT in AH and AAH phase. It suggest that the combination application of OMT+ATRA orOMT+Se in hepatocarcinogenesis could inhibit the expression of TERT to some extent, delay the progressof hepatocarcinogenesis, improve the survival quality and prolong existence.