Studies On Toxicology Of A Drug Pair Of Prescription-Salviae Miltiorrhizae And Veratrum Nigrum And Electrochemical Behaviors Of Its Active Ingredients

"18incompatible herbs" is an important part of Chinese medicine incompatibility. It is one of the most controversial issues of modern herbal theory. Most of the ancient medicine strictly follow "18incompatible herbs", but there are few scholars believe that the "18incompatible herbs" are not strict incompatibility. At present, studies of "18incompatible herbs" is increasingly great attention. And the results of their research is also increasingly richer. Salviae Miltiorrhizae (SM) cooperating with Veratrum Nigrum (VN) is a drug pair of prescription incompatibility of the "18incompatible herbs". In the present study, based on Chinese herbal medicine theory toxicity studies, rat toxicology experiments as a research platform, and combined with electrochemical means, in the body fluid conditions, research the relationships of SM and VN compatibility and toxicity. The main contents include the following aspects:1. By electrochemical methods, electrochemical behaviors of different mass ratio of SM and VN extraction was investigated by cyclic voltammetry and differential pulse voltammetry at a bare glassy carbon electrode. When the mass ratio of SM and VN were1:0,10:1,5:1,3:1,2:1,1:1,0:1, cyclic voltammograms and differential pulse curve were measured in pH7.4phosphate buffer solution. Plotting the peak current value to quality of VN, when the mass ratio of SM and VN is5:1， the peak current changes greater, suggests that this mass ratio may have a greater toxicity.2. SM extraction, VN extraction and mixture extraction of SM and VN (5:1) were administered to male Wistar rats by gavage for15days, respectively. The survival status of the experimental animals, organ index, serum levels of ALT, LDH, CK,BUN and CREA were compared with the blank control group. When rats were administrated mixture extraction of SM and VM, increased levels of serum creatine kinase (CREA) as well as decreased levels of serum blood urea nitrogen (BUN) were shown. Meanwhile the relative kidney weight were decreased. These data suggested that the combined use of SM and VN could involve in the toxic effects in kidney of rats.3. Protein content and total P450were determined when SM extraction, VN extraction and mixture extraction of SM and VN (5:1) were administered to male Wistar rats by gavage for15days, respectively. Results from our experiments, CYP450content were decreased dramaticlly in the groups of treated by mixture extraction of SM and VN rats. The results suggest that cooperating of SM and VN might have an impact on metabolism, further inhibition of the metabolism of toxic components.4. The electrochemical behaviors of salvianolic acid B was investigated by cyclic voltammetry at a bare glassy carbon electrode, and differential pulse voltammetric method for the determination of salvianolic acid B was developed. In phosphate buffer solution between pH2.0-9.0, salvianolic acid B exhibited a pair of reversible redox peaks at bare glassy carbon electrode. At optimal experimental conditions, the relationship between oxidation peak current and the concentration of salvianolic acid B was linear in the range of1.95×10-7～3.90×10-6mol/L, and the detection limit was1.30×10-7mol/L (S/N=3). The average recovery rate was101.1%(RSD=1.3%). This method was successfully applied to the determination of salvianolic acid B in complex prescription salviamiltiorrhiza tablets with satisfying results.