Research On The Role Of FADD Gene In Inducing Apoptosis Of Bovine Ovarian Granulosa Cells

Recently，the beef industry is in a rapid development of China. It has a choserelationship whether beef is good to its economic benefits. Thus, it is necessary tostudy the gene and protein which associated with cattle reproductive performance.FADD, Fas associated protein with death domain, is cloned from yeast two-hybridsystem, and bind with Fas molecule cytoplasmic through DD, induce Fas/FasLpathway in turn related to induce apoptosis. FADD has an important role in theprocess of apoptosis-related, therefore, it is considered to be a molecule of apoptosis.FADD can play an important function in signal transduction of cells and plays acrucial role in apoptosis, embryonic development and inflammatory reaction in thebiological activity. Although scholars have researched in further deepening of FADDin China and abroad, but related research reports about bovine FADD gene is not somuch, therefore, whether FADD gene can be used as genetic markers of cattle meatand beef cattle ketones traits and in breeding traits require further study andinvestigated.RNAi is a technology of using a short double-stranded RNA synthesised in vitroto inhibit specific gene expression, is a powerful tool for studying gene function ofpost-transcriptional gene silencing. It has great significance of the discovery of RNAi,not only reveals the depth of intracellular gene silencing mechanism, but also is thepost-genomic era gene function analysis, greatly contributed to the progress ofmankind reveals the mystery of life. More and more researchers began to use RNAitechnology to study gene expression.Therefore, this study is about FADD gene, isolated and cultured ovariangranulosa cells from the local cattle as a research material. FADD gene RNAi vectorand overexpression vector as a control study was constructed, and were transfected into granulosa cells. Respectively, Real-time quantitative PCR and western blottechniques were used to detect FADD gene mRNA and protein changes, and flowcytometry and apoptosis detection kit were used to detect apoptosis change. Theresults confirmed the FADD gene overexpression promote granulosa cell apoptosis,while cells inhibit apoptosis was transfected FADDRNAi carrier, It further illustratesFADD gene expression affect ovarian granulosa inducing apoptosis, and lay thetheoretical foundation for the improvement of the reproductive performance of beefcattle.