Trametes Versicolor Polysaccharopeptide (PSP) Modulated TNFR1 Pathway During The Apoptosis In Molt-4 Cells

Polysaccharopeptide (PSP) is a bioactive component derived from Trametes versicolor COV-1 strain deep-layer cultivated mycelia. As a new medicine, PSP is used to enhance immune functions and agonize side effect by tumor patients during the chemo- or radio-therapy. This study was to investigate modulation of TNFR1 death receptor pathway by PSP during the apoptosis on human leukemia cell Molt-4.Results of transmission electron microscope showed 400?g/mlPSP caused the typical character of apoptosis such as chromatin condensation, plasmic contraction, apoptotic body and so on. The date of flow cytometry revealed that the Positive Gate (PG) of TNFR1 was increased 0.32%,4.08%,11.27% respectively, when Molt-4 cells were incubated with 25,100,400?g/mlPSP; When Molt-4 cells were incubated with 400?g/mlPSP for 12,24,36 or 48h, the PG was increased 5.71%,36.48%,37.25%,40.88% respectively. Cells incubated with 2.5?g/100?l TNFR1 monoclonal antibody were treated with or without PSP, and then TNFR1 monoclonal antibody could antagonize 16% cell inhibition caused by PSP. In the experiment of ELISA, PSP didn't modulate the expression of TNF-???Immunoprecipitation was used to detect the content of TRADD, and the result hinted PSP promoted the combination of TRADD and TNFR1. Immunofluorescent staining of FADD molecule demonstrated that it was enriched at the inner side of the cytoplasm membrane. The analysis of molecular Bid by western bolt revealed that PSP didn't connect TNFR1 death receptor pathway with mitochondrial pathway by Bid. These findings implied that PSP modulated TNFR1 death receptor pathway during the apoptosis in Molt-4 cells.