Study On The Germination Characteristics And The Technology Habituation Of Bletilla Striata

Bletilla striata (Thund.) Reichb.f is a genus of herbaceous perennial flowers. Bletilla not only has high ornamental value, but also is one of our traditional Chinese medicines, its bitter, sweet, astringent, cool, lung, stomach, liver. Functional convergence bleeding, swelling and tissue regeneration, treating pulmonary bleeding, traumatic bleeding, carbuncle swollen sore, chapped skin, burned. With the increasing demand of Bletilla, resulting in wild Bletilla and excessive excavation; as the same time,the destruction of natural habitats, but also exacerbated the reduction of wild resources, wild Bletilla on the verge of extinction. Tending this resourse should be urgently neededly. In this paper,based on its seeds and plantlets as materials, investigate the seed germination characteristics under natural conditions and germination of symbiotic bacteria on tissue culture, and how to improve transplanting survival measures.Provide the theoretical basis for solving the above problems.In order to know the natural seed germination characteristics of Bletilla, establish the technical of germination, we used the original habitat planting methods, explore the different cover on seed germination, summed up the natural seed germination characteristics and properties of natural death of Bletilla, and for different sowing methods on seed germination rate and germination and growth indices were also some analysis. The results show that in the natural, if you choose winter sowing, the general approach to select the film cold. If the condition is not afford it, you may also overburden and Mulching way, As far as possible more than15cm, turns the soil covering grass at least9cm.In order to study the relationship of the mycorrhiza and the seed germination, the paper collected by the original habitat of the Bletilla and sowing the seeds grow into seedlings germinate, hyphae group using a single separation method of seed endophytes, the results have not been isolated to Portland plants of the dominant groups Rhizoctonia fungi, but isolated in the early gennination more Fusarium, Neonectria, Phomopsis and so on. This article from different substrates, affecting the way out of the bottle, the means of hormone, and different forms of tissue culture for transplanting survival rate, the results show that the complex mix of cultivated garden soil matrix, placed in a six-day outdoor plant seedlings the best survival rate, but also during the growth, the seedlings watered with nutrient solution is helpful. It can be seen through the test with mycorrhiza, endophytes promote dialogue and does exist, inhibition and no effect three states, BJQX106on stem diameter, corm size and total root length has a role in promoting; BJ13203on plant height, leaf length, stem diameter and total root length also has a role in promoting.Appropriate carbon source and level of seed germination and seedling growth were selected to shorten tissue culture time of Bletilla striata. It chose three carbon sources (glucose, sucrose and soluble starch) and two levels (30g/L and60g/L) were choosed for randomized block design. Statistics analysis of seed germination and seedling growth were used to select appropriate carbon sources and their levels. Results The results showed that glucose and soluble starch promoted the embryo enlargement and greening and no significant between levels, sucrose increased plant height, the lengthen and the width of leave,fresh weight, and root length significantly. The conclusion is that30g/L soluble starch was used for seed germination and sucrose for seedling growth.In order to investigate the effect of the natural environment on survival rate of transplanted tissue culture, and hereby choose Bletilla original habitat and other orchid plants, Fangxiang Township is from Guizhou province. As so far, there are no reports about Bletilla transplanting plantlets in nature. This paper uses the transplanting native habitat, explore the different months of the fresh weight of transplanting, transplanting survival rate, longitudinal diameter, affecting diameter, corms area, root number and total root length and other aspects. The results show that the relative in August and September and October and July, July is the best time of transplanting, July transplanting tissue culture in fresh weight, height, total root length and other indicators are the best.