Red-eared Turtle Erythrocyte Energy Metabolism Related Research(Trachemys Scripta)

Blood is the body’s lifestring and erythrocyte is one of the main substance in blood. Erythrocyte’s surviving, aging and oxidation is closely related to erythrocyte’s ATP content. We hope to know more about the energy metabolism of the erythrocyte at low temperatures during diving. However, research data about the Red-eared turtles composed of erythrocyte at low temperatures diving, and other aspects of energy supply is still very lacking. Special physiological structure of erythrocyte and resistance to low temperature stress tolerance of hypoxia of Red-eared turtles will be discussed in this study. We hope to be able to better understand the underlying mechanism of red-eared strong resistance to energy metabolism and its relationship with the energy metabolism of erythrocyte.So we designed a week of diving hypoxia with low temperature experiment to study. In this experiment, a lot of energy metabolism related data Indicators, providing a reference for subsequent research work, have been measured. We found that turtle’s glycogen did not change significantly, while blood glucose levels increased by about20%in the seven-day experiment. Meanwhile triglycerides is about six times higher than the normal state。 And then we compared the data related to energy metabolism in humans and rats with turtles. First, Human serum ATP preservation with EDTA is lot better than red-eared turtles’ or rats’. Second, Energy requirements for red-eared turtle diving at low temperature may rely mainly on fat, but easily depleted glycogen. Third, Lactate and pyruvate ratio for red-eared turtle diving at low temperature is very stable in vivo may be closely related with high concentrations of lactate dehydrogenase and elevated calcium and magnesium ions concentration. Forth, Pyruvate and lactate in serum may be associated with the erythrocyte inside surface and outside ATP concentration.The study in this paper provided experimental data with important clinical significance and value. This paper provided the basis for further studies of energy metabolism, low temperature tolerance and anti-hypoxia response mechanisms.