Large-scale Clonging And Preliminarily Functional Analysis Of The Rice Blast Fungus Secreted Protein Genes

Due to its significance in agricultural production, as well as the availability for genetic manipulation, genome sequence information, the rice-Magnaporthe oryzae system has become an important model for researchings on plant-fungus interaction. Study on isolation and functional analysis of M. oryzae effector proteins is one of the important research directions for rice-M. oryzae interaction. Genome-wide predictions revealed that the rice blast fungus produced more than1000secreted proteins. Many of the secreted proteins play as functional effectors with important roles in the process of rice blast fungus infection of rice. However, the function of the majority of M oryzae secreted proteins has not been experimentally identified.In a previous study based on high-throughput transcriptome analysis study, a total of851M. oryzae secreted protein genes were identified as in-planta expressed genes. By protoplast transient expression assay, and agroinfiltration of Benthmiana nicotiana leaves, five effector proteins that induced plant cell death were identified from42candidate in-planta expressed M. oryzae secreted protein genes. In this study, we further use prokaryotic expression system to research the five effectors for toxicity domain, and adopt chemical inducible expression system to investigate the effect of the five effectors in rice. Meanwhile, a total of101new M. oryzae secreted protein genes were cloned into a plant expression vector, providing important resources for identification and functional analysis of M. oryzae effector proteins.