| Dermatophytosis was a common infectious disease in small animals. It was commonly caused by superficial mycoses, one of the common diseases in dogs and cats. With the rapid development of medicine technology and resistance of fungi, clinical recurrent rate also increased recently. At the same time, a large number of antifungal drugs were applied in clinics, resistant fungis had become dominant fungis, which could induce gene mutations, lead fungus to growing drug resistance, making treatments more difficult. These kind of fungus were basically grown in hair, skin and nails by parasitization or saprophytism in the surface stratum corneum, the keratin of hair and nail plate. Superficial mycoses included pathogenic dermatopbyte (M. canis, M. gypseum, T. mentagrophytes and E. floccosum. etc), conditional pathogenic candida (C. albicans, C. tropicalis and C. parapsilosis. etc) and some of the non-pathogenic fungi (penicillium and aspergillus).The separation, cultivation and identification of common fungus in clinics, included M. canis, M. gypseum, T. mentagrophytes and E. floccosum etc, and had provided scientific basis for diagnosis and treatment to skin disease of clinics by means of collecting clinical samples.89cases of canine dermatosis were collected in a period of6months. Among them, cases of mites were23, accounted for25.8%, fungal patients were45, accounted for50.6%. Among all skin diseases, M. canis dogs were19.1%. M. gypseum ones were16.9%and malassezia were5.6%. Among all the fungal diseases, M.canis and M. gypseum accounted for71.1%, indicating that M. canis, and M. gypseum were major fungal pathogens of dermatophytosis in dogs.The artificial infection model of Microsporum canis was established by using fungal strain isolated from clinical sample. Conditions, such as the spore liquid concentration and the best time for model establishment were explored. The result indicated that105mL-1concentration of macroconidium liquid was better than other concentrations. The skin inoculated began to appear erythema3days after inoculation and appeared obvious pustules and ulceration, and spreaded to the whole body after2weeks The most serious skin damage occured when inoculated after6-8weeks. Then after inoculation with12-14weeks, the skin lesions began healing gradually from the center and heal finally. This model experiment provided help and guidance to drug test of the local and systemic treatment of fungal infection.This experiment also observed the treatment effectiveness of terbinafine hydrochloride to the fungal skin disease infected with M. canis, in cilinical dogs. The result showed that recommended dose (4cm2,2times per day, for4weeks) of terbinafine hydrochloride is good. The cure rate and obvious treatment efficience of the recommended dose group and the double dose group were higher than85%(P<0.05) compared with the control group. After first weeks, the total effective rate was more than85%, compared with the control group with extremely significant difference (P<0.01). By second weeks, the total effective rate reached100%. Although clinical symptoms disappeared, the drug should be administrated again for2more weeks, ensuring that the fungi was completely eliminated, dicreasing recurrence rate. Based on calculation of scores of clinical signs, both terbinafine hydrochloride and ketoconazole had good treatment effectiveness on fungal infections. First weeks both cure rate had no significant difference (P>0.05). From the beginning of the second week, terbinafine hydrochloride effective rate was better in compound fluconazole, the differences between them were significant (P<0.05). It was indicated that terbinafine hydrochloride has good curative effecttivness, short therapeutic course and was worth to be popularized in clinic application. |
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