Effedts Of Paclobutrazol Exposure On Antioxidant System And Disrupts Spermaotgenesis Of Sebastiscus Marmaratus

Paclobutrazol (PBZ) is widely used as triazole and plant growth regulator in agriculture. butthe toxicology of PBZ on fishes was seldom studied. This search was designed to detect theinfluence of PBZ on the spleen antioxidant system of Sebastiscus marmoratus that was exposedat an environment concentration(10,100,1000ng·L-1) of PBZ for50d. The result showed that theglutathione (GSH),glutathione-S-transferases (GST),glutathione peroxidase (GSHPx),malondialdehyde(MDA) of spleen， liver and brain were presented with a concentrationdependence variation ander the action of PBZ, which indicates the exposure of PBZ had harmfulto the antioxidant system of Sebastiscus marmoratus. The result showed that long-term usage ofPBZ may cause ecological pressure on aquatic organisms such as fishes and aquatic environmentetc. In view of the long-term used of pesticides that lead to environmental problems,it isnecessary to carry out the researches that pesticides have toxicogenic effect on fishes andMarine organisms.This study has actual significance for the protection, estimate and monitor ofthe ocean ecological environment.The present study was conducted to investigate the effects ofPBZ at environmentally relevant concentrations on testicular development in male rockfishSebastiscus marmoratus and to gain insight into its mechanism of action. After exposure to10,100and1000ng/L PBZ for50d, although the gonadosomatic index did not show a significantalteration, there was a reduced number of mature sperm and an abundance of the late stages ofspermatocysts in the testes. The activity of γ-glutamyl transpeptidase (which is used as aSertoli cell marker) was decreased in a dose-dependent manner after PBZ exposure, which mightbe a result of the reduction in FSHβ expression in the brain. The toxicity induced byaccumulation of PBZ in the brain might be primary cause impacting the spermatogenesis viabrain–pituitary–gonad pathway.