Isolation And Identification And Biological Characteristics Of Pseudomonas Aeruginosa Isolted From Chicken

In this study,14strains of bacteria isolated from the diseased chicken flocks as research object, thebacterial biochemical identification, drug sensitivity test,16S rRNA sequencing and pathogenicity testsare carried out on it, and lay the foundation for further study on the variation of Pseudomonasaeruginosa threats and conditions on poultry pathogens.The present study from infected chicken this research is divided into three parts.The first part: the chicken of Pseudomonas aeruginosa isolated and identified by microbiologymethod of Handan, Hebei, Hengshui, the incidence of chickens of different etiology study. Asepticcollection disease disease of chicken feed, using a variety of biochemical method to separate pathogenicbacteria were identified. Drug sensitivity test to separate pathogenic bacteria, drug sensitive test resultsshow, the multiple drug resistance of Pseudomonas aeruginosa in Hebei province is very serious, thehighest up to18high altitude.24kinds of drug susceptibility of choice, only to levofloxacin andpolymyxin B is sensitive to100%.The second part: the sequence of chicken16SrRNA gene of Pseudomonas aeruginosa16S analysisusing PCR technology to chromosomal DNA of Pseudomonas aeruginosa as template. And thensequenced and analyzed its, test results show that, specific amplified band is consistent with theexpected size, and the detection method has a good specificity, high sensitivity.The third part: Pseudomonas aeruginosa was purified by separating the animal pathogenicity testwere analyzed by pathogenicity tests on chickens, mice, rabbits in groups, test proved wereintraperitoneal injection and intramuscular injection in mice, rabbits caused by animal morbidity andmortality of chicks and chicks, not seen in animal disease caused by oral administration. The death ofchicks subcutaneous edema fluid, muscle, blood, liver, blood and death of Mice Ascites, death rabbitheart, liver, spleen, stomach were recovered to Pseudomonas aeruginosa. That high incidence inchickens, mice, rabbits and the bacteria.