Survery Of The Sheep-borne Hard Tick Species On Sheep And Tick-borne Anaplasma,Piroplasma Carried In Henan Province

Tick is an important medium for pathogen transmission, and about10%ofthe ticks can carry pathogens, which cause different diseases in livestock populations.Different kinds of hard-ticks are responsible for the dissemination of different pathogens.Ticks can lead to a variety of disease outbreaks in sheep flock which are great threat tosheep industry. So, it is very significant to unveil the tick-borne disease of sheep with theresponsible dominant tick species. Anaplasmosis and Babesiosis are common bloodparasitic diseases of sheep carried by the ticks. This study was the first initiative toinvestigate the popular ticks of pathogenic importance in sheep in Henan province, whichwould provide a reference and theoretical basis for the study of tick-borne diseases later on.This experiment identified a total of386sheep-borne hard-ticks from the sheep of20different cities of Henan province during the period from July2011to September2012.Optical microscope was used to describe the morphology and determine the tick speciesaccording to the historical data. Conventional PCR technique based on both small ribosomalsubunits (16S rDNA) and Internal Transcribed Space2(ITS-2) was used to carry onsequence analysis and identify the species and genotypes of the sheep ticks in Henanprovince, and also to determine the molecular evolutionary relationship between the sheeptick in Henan province with similar hard ticks species which had reported in Chinapreviously. MEGA, Lasergene sequence analysis software and so on were used to comparethe hard-ticks sequences in different regions of Henan province. The relationship betweenpopular tick species and different areas of Henan province was also analysed. The resultshowed that the sheep-born tick species collected from20cities of Henan province was allH.longicornis. The BLAST result of16S rDNA gene sequence showed that the homologywith H.longicornis (JF979373.1) from LanZhou plants was99.9%,99%with SiChuan(JF979373.1) and HeBei (JF979374.1) plants, E value was0.1. The ITS-2gene sequenceBLAST result showed that the homology with H.longicornis (JF9793-73.1) fromHeBei(JQ346683) plant was99%, E value was0. Selected26(Each region1-2)16S rDNAsequences from the sequencing result carried on the difference and homology analysis.According to the result, the differences between the26sequences were small and there wasa few minority basic group substitution or missing. Sequence homology analysis result showed that the homology between the largest value was100%whereas the minimum valuewas82.7.To investigate the prevalence of Anaplasma and Babesia in hard-ticks in Henanprovince, a total132DNA samples were tested by normal PCR and nested PCR technology.The PCR result determined that34samples were infected with Anaplasma and the positiverate was22.7%where3samples were infected with A. ovis, the positive rate was2.3%;27samples infected with A. bovis, the positive rate was20.4%;4samples infected with A.phagocytophilum, the positive rate was3.0%;1sample infected with A. Bovis and A.phagocytophilum, positive rate was0.76%; T. luwenshuni positive rate was14.4%, B.motasi positive rate was0.75%. The research showed that the dominant tick species was H.longicornis, which could carry pathogens commonly. Notably, the zoonotic pathogen, A.phagocytophilum was detected in the samples. So, the control of tick-borne diseases shouldbe enhanced.The research showed that the dominant tick species was H.longicornis, which carriedpathogens is common. Especially, the zoonoses pathogen of A.phagocytophilum wasdetected in the samples. Apparently, the control of tick-borne diseases should be enhanced.According to the three aspects of controling the source of infection, protecting animalinfectibility and cutting off the pathogen transmission, animal heath was protected. This is aprotection for human health and animal husbandry.