Studies On The Strains New High-potassium Tobacco By Inducing Mutation With Ion Implantation

In this study, potassium and efficient genotype New Tobacco Strains test material by ion implantation processing then planting, breeding quality traits from the M2generation, continue to plant disease resistance improved plant after harvest. Determination of selected M3generation transgenic tobacco seedlings quality of the activity of the seedling stage SOD activity, CAT activity, POD activity, etc. record M2field of character variation, agronomic traits and disease occurrence; SRAP molecular marker technology and the use of bacterial wilt disease resistance gene identification, radiation treatment samples difference with the original strains studied at the molecular level.The main results are as follows:1.Ion implantation processing the M3generation tobacco seedling quality than control lines GK2、GK5into seedling root fresh weight、root to shoot ratio exists a very significant difference, stem height、stem girth、the effective number of leaves, root dry weight、fresh weight of stems and leaves, stems and leaves dry weight were significantly different; transplanting of stem height there are significant differences, there were significant differences in stem girth, other traits were no significant differences. M3generation of GK2(10) stem height increase of22.14%, stem girth increase of15.13%,22.58%increase in the effective number of leaves, root fresh weight increased by24.72%,57.14%of the root dry weight increase, stems and leaves of fresh weight increase of7.89%, stems leaf dry weight increase of15.38%GK5(18) stem height increase of15.54%, the stem girth increase of6.71%, an increase of5.56%in the number of effective leaves, root fresh weight increase of11%, stem and leaf fresh weight increase of4.93%, stem and leaf dry weight increase of8.93%.2. Ion implantation processing changes in the M3generation strains physiological features. By ion implantation processing M3generation of SOD, CAT and POD activity compared to the control lines significantly enhanced, the GK2(9) than the GK2strains SOD activity increased44.2%, CAT activity increased13.9%, POD activity enhanced by40.9%; GK5(18) than the GK2strains SOD activity increased95.0%, CAT activity increased89.3%, POD activity enhanced by50.0%.3. Ion implantation M3generation of strains camparison to strain GK2、GK5original species improved. The generation M3on behalf of GK2(9) as compared to the control lines GK2bacterial wilt incidence of reduced53%, the M3generation GK5(18) compared to the control lines bacterial wilt incidence of62%in GK5. Ion implantation processing the high potassium strains bacterial wilt disease resistance played a modified.4.220pair of forward and reverse primers screened, which could amplify a specific band of the M3generation lines and control lines. The Mel-Em land Mel-Em5primers can detect the field performance of bacterial wilt resistance and improved generation M3strain specific band control lines can not detect a specific band, Me2-Em4and Me7-Em5GK2strains can detect a specific band, and GK5strains can not detect the specific band, SRAP molecular marker technology can detect GK2on GK5strains molecular level differences and the bacterial wilt disease resistance at the molecular level the difference.