The Change Of The Incidence Of Fatty Liver During The Peripartum Period Of Cows And The Preliminary Exploration Of Biomarker In Serum

This experiment has passed two parts. The first one is to explore the prenatal fatty liver changes in blood parameters,determining the incidence of suspected fatty liver and then through thesensitive detection of serum markers to identify a possible biomarker fatty liver.Part I: Serum samples were collected respectively28days,21days,14days,7daysbefore the expected date, and postpartum2days,9days,16days,23before early feeding todetermine NEFA, Glu and AST. NEFA was found to reach the highest level the next day afterdelivery and had kept a high level after childbirth. Glu was at a high level before childbirth,which began to decline from the7days before delivery and reached a low level afterchildbirth. AST was at a low level before accouchement, which increased7days beforedelivery and reached its maximum value at9days after parturition. Suspected incidence offatty liver reached the maximum value (66.48%) in the first16days after delivery, and thesuspected incidence of fatty liver in various periods after childbirth of cows that had morethan three fetal are lower than the cow groups under three fetal.Part Ⅱ: liver and serum was collected from the cows three months postpartum.According to the proportion that triglyceride in wet weight liver，cows were divided intonormal group and the fatty liver group. Measured phospholipase A2，fibroblast growthfactor-21, hemoglobin α, hemoglobin β,1,25-dihydroxyvitamin D3by ELISA in serum, weanalyze the difference between them.The results showed that the significant differencesbetween fibroblast growth factor-21and hemoglobin β were P values,0.0269and0.0312respectively. We conclude that these two proteins may be used as cow serum fatty liverbiomarkers. Fibroblasts growth factor-21and hemoglobin β were adjusted up or down,according to the rate of misdiagnosis and missed diagnosis rate, we determined the criteria offatty liver diagnostic were1091.91ng/L and3.03ng/L. Liver triglycerides and serumfibroblast growth-21significant positive correlation, but the correlation coefficient is only0.4939. Experiments were fitted fibroblast growth factor-21, hemoglobin β,lipoprotein-associated phospholipase A2three kinds of indicators and liver triglycerideregression equation, fibroblast growth factor-21, lipoprotein-associated phospholipase A2two kinds of indicators and liver triglyceride regression equation, Fibroblast growth factor alone and liver triglycerides-21regression equation. These three equations R2values werelow, and they were0.3328,0.3229and0.2823respectively.