Splenic Gene Expression Profiling In White Leghorn Layers Infected With The Salmonella Enteritidis

Salmonella is the Enterobacteriaceae of negative of coloring of change orchid familyname. It has caused great harm to layer and food safety. Layer salmonellosis is a chronicdisease caused by Salmonella. Although the disease is not fatal, it will lead to the laying rate.After a certain period of time, the disease can cause the layer gastrointestinal dysfunction andthe chickens decreased resistance to disease and then infected with other diseases. Somespecies of Salmonella have the genetic plasmids carrying resistance factors. It is hard to treatsalmonellosis for resistance factors with resistance to multiple antibiotics. Terms of chicken,it is one of the effective ways to reduce the laying hens harm caused by salmonellosis ofstudying molecular mechanism of resistance to Salmonella infection. The study is open layeras the research object. Collection of blood infected with SE, and then detection of bloodphysiological indicators. In this study, we use the4×44k chicken V2cDNA microarray toscreen the differently expressed genes in infected and non-infected chicken after8days and16days. Subsequently, the analysis of clustering and enrichment of biological process andmolecular function and cellular component of gene ontology as well as the pathway wasperformed using DAVID database. In addition,11differently expressed genes were selectedfor validation of microarray results by QRT-PCR. Statistics immune-related and the samegenes contained in the differentially expressed genes at two time points. The results showedthat:1) The number of leukocytes in the blood sample is generally higher than the normal, butthere was no significant difference among the groups;8days after infection, the test groupnumber of red blood cells was significantly higher than the control group, but there was nosignificant difference at16days after infection.2)11differently expressed genes were selected for validation of microarray results byQRT-PCR. We do the correlation analysis with the results of gene chips and QRT-PCR andget the correlation coefficient0.804. The result of gene chips and QRT-PCR is consistent, sothat the gene chips obtained in the present study results are accurate and reliable. 3) There were1371differentially expressed genes in spleen tissue of open production ofWhite Leghorn chickens after infection8days with SE, meanwhile, there were692differentially expressed genes after infection16days.4) Cluster analysis showed that chicken of the same point in time were initially clusteredtogether because their expression profiles were similar.5) GO and Pathway analysis showed that they were mainly involved in GO biologicalprocess of G-protein coupled receptor protein signaling pathway, embryonic gut development,immune response, leukocyte adhesion, and the KEGG pathways of Cell cycle, Neuroactiveligand-receptor interaction, Steroid hormone biosynthesis, Cell adhesion molecules(CAMs),Retinol metabolism and so on.The results will further deepen the understanding of the molecular mechanism of thechicken Salmonella infect, provide a theoretical basis and scientific basis for the chickendisease molecular genetics and breeding.