Analysis Of The Cecal LncRNA Expression Profile In SPF White Leghorn And Jining Bairi Chicken After Salmonella Enterica Serovar Enteritidis Infection

Long non-coding RNA(lnc RNA)is the length of more than 200 nucleotides of non-coding RNA,which plays an important role in the genetic imprint,chromatin remodeling,cell cycle regulation,splicing biological functions such as regulat on,m RNA degradation and translation regulation.Recent studies have shown that lnc RNA aslo plays an important role in bacterial and viral infections.Salmonella enteritidis is a foodborne pathogenic bacteria,which has important significance in public health.It causes severe intestinal inflammation mainly by attactking intestinal mucosa.The genetic background of the host plays a key role in chicken resistance to salmonella enteritidis infection,and there are significant differences in infectibility to Salmonella enteritidis among different breeds of chicken.In recent years,high-through put sequencing technology has become a powerful tool for lnc RNAanalysis and identification of different species.At present,there are applications in bacteria and virus infected chicken.The regulatory mechanism of lnc RNAin salmonella enteritidis infection in chicken remains unclear.In this research,we used salmonella enteritidis to infect white leghorn chickens and Jining Bairi chickens that had just emerged from shell for three days.We collected cecum samples on the seventh day after the infection.The control group and treatment group respectively to build six small RNA libraries.They are control groups: SCI,SC2,SC3,JC7,JC8,JC9 and ST4,STS,ST6,JT10,JT11,JT12,totally 12 libraries.The lnc RNAwas identified by high-throughput sequencing technology to identify the differences in salmonella infection after enteritidis infection.And the sequencing results were verified by fluorogenic quantitative PCR.At the same time,the expression of lnc RNAand target genes in white leghorn chicken and Jining Bairi chicken's cecumtissues were analyzed by fluorescence quantitative PCR.The results showed:(1)Through high-throughput sequencing technology,screening was performed in 12 libraries,including SCI,SC2,SC3,JC7,JC8,JC9 and ST4,ST5,ST6,JTIO,JTII and JT12.Clean datum respectively are 78,192,676 G ? 70,738,170 G ? 77,276,380 G ?79,242,068G?69,736,056G?75,090,818G?71,655,498G?66,067,530G?76,768,730G?77,237,062G?79,352,414G?77,197,824 G.(2)Based on the results of bioinformatics data detection,according to the two standards of p<0.05 and |log2(Fold change)|>1,we were in SPF white leghorn chickens infection group and uninfected group with text description,infection and control white leghorn chickens comparison(SC,vs ST),in Jining Bairi chicken infection group and uninfected group(JCvs JT)group,SPF white 736,673,224,and 90 differentially expressed genes were screened in the white leghorn chickens infection group and the Jining Bairi chicken infection group(STvs JT)group,the uninfected group and Jining Bairi chicken uninfected group(SCvs JC)group.Then,these differentially expressed genes were annotated by functional annotation.It is found that multiple signaling pathways are related to immune response,such as congenital immune response,adaptive immune response,apoptosis and autophagy.(3)In the SCvs ST group,13 differentially expressed lnc RNA were selected,6differentially expressed lnc RNA were selected in JCvs JT group,11 differentially expressed lnc RNA were selected in SCvs JC group,and 2 differentially expressed lnc RNA were selected in the STvs JT group.The results showed that lnc RNA was associated with 135 target genes,including 7 immune-related genes.Significant enrichment in Biological_Process pathways are cellular process and macromolecule metabolic process.Significant enrichment in Cellular_Component pathways are cell part and intracellular part.And significant enrichment in Molecular_Function pathways are binding,heterocyclic compound binding.(4)Identifying 18 key lnc RNA difference is extremely significant.These lnc R NA participate in the biological processes of inflammatory response,immune syst em development and lymphocyte activity.The relationship between lnc RNA and t arget genes was affected by the genetic background of lnc RNA in the White Leghorns and Jining Bairi chicken.We found 9 lnc RNA associated with salmonella e nteritidis,corresponding to 109 target genes.They were significantly enriched in Cytokine-Cytokine receptor interaction,RNA degradation,and m RNA surveillanc e pathway,etc.In the end,we found that ENSGALT00000078841,ENSGALT00000087884,ENSGALT00000069943,ENSGALT00000083946,ENSGALT00000086955and other lnc RNA.They played an important role through target gene CCL19,C LP1 and TIMM10 after enteritidis salmonella infection.(5)This study lays a foundation for intensive study of the mechanism of lnc RNA in enteritidis salmonella infection and provides theoretical and scientific bas is for molecular disease resistance and genetic breeding of chicken.