Screening Genes For Muscle Of Rabbit By CDNA Microarray

Rabbit meat featuring high protein, lecithin, amino acid and low cholesterol, commonly known as the "three high and one low", is the ideal meat food for obesity, liver disease, cardiovascular disease and diabetic patients. Although the rabbit meat is a kind of high quality meat food, popularization is relatively low due to the restriction of eating habits and rabbit feeding and management. At present, domestic research on rabbit meat quality traits is far less than the pork, poultry, which is also an important factor of restricting the rabbit industry development. This research selected New Zealand white rabbit and Fujian yellow rabbit as the research object, in order to explore the differences of muscle performance and find out the rabbit muscle development molecular mechanism, aiming to provide theoretical basis for the improvement of meat quality traits and certain reference for meat rabbit breeding work.This study analyzed the muscle differences between New Zealand white rabbits and Fujian yellow rabbits using microarray, fluorescence quantitative PCR and PCR-SSCP. The main results are as follows:1. This research screened out different expressed genes in different parts of New Zealand white rabbits and Fujian yellow rabbits,including Myh6, Myh7, Myh7b, Myo5b, Tnnc1, Tpm3, Acta2and so on, which are all closed related to muscle development and the level of gene differentially expressed at least3times.2The results of fluorescence quantitative PCR showed that the expression pattern of Tpm3and Myh7genes were similar. The overall expression level in dorsal muscle than in leg muscle. Similar time and space of two genes they may showed they have synergistic effect.3. In this research, primers of the fifth exon were established to analyze genetic diversity of Myh7gene firstly. The results were that the total length of the fifth exon was109bp, but we amplified145bp including the whole exon and part introns. We found47AA and5AB genotype in Newzealand white rabbit,52AA and14AB genotype in Fujian yellow rabbit, there is no BB was found in both2groups. In Newzealand white rabbit, the gene frequency of A(non-mutation) is0.952and B(mutation) is0.048, while A(non-mutation) is0.894and B(mutation) is0.106in Fujian yellow rabbit. Sequencing and alignment revealed that it occurred single base mutation in2375site, where the original thymine (C) was changed into thymine in few individuals. Sequence analysis and codon comparison showed this mutation maked no change in amino acids, which is synonymous mutation. The two level structure of Myh7protein containing1935amino acids, which including alpha helix (h)74.94%, random coil (c)19.17%, extension chain (e)5.89%. Myh7protein contains two helical chains with no complex interweave, and the chain-like Characteristics may related to structure of myofibril.