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Progesterone Receptor (PR) And Estrogen Receptor(ER) Gene Expression And The Effects On Ovary Tissue Of High-quality Chicken In Monochromatic Light

Posted on:2014-08-07Degree:MasterType:Thesis
Country:ChinaCandidate:L B LiuFull Text:PDF
GTID:2253330425451455Subject:Animal breeding and genetics and breeding
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Artificial illumination is an important exogenous factor in the control of many physiological and behavioral processes and also an important factor in the management of layers. The aim of this study was to examine the effect of monochromatic light on gene related reproduction, fertility and quality of eggs of China local mountainous laying hens. In our research, Erlang Mountainous chickens, one cultivated breed, were used. Light treatments were:control white (400-760nm), blue (480nm), green (560nm), yellow (590nm) and red (660nm). We collected data about the effect of monochromatic light on reproduction traits, adopted real-time RT-qPCR (SYBR green I) method to evaluate the expression of PR, ERa and ERβ mRNA, and used Western Blotting analysis evaluate the expression of PR-A and PR-B protein.Results showed that monochromatic light hadn’t effect on age at first egg (P>0.05); extremely significant effect on body weight at first egg (P<0.01), blue light significantly higher than red light; significant effect on egg weight at first egg (P<0.05), blue light was highest. Monochromatic light hadn’t effect on Egg production in300days and Body weight in300days; but extremely significant effect on egg weight in300days (P...
Keywords/Search Tags:laying hen, monochromatic light, progesterone receptor, estrogen receptor, ovary, follicle
PDF Full Text Request
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