Isolation Of Glutenin Subunit And Cloning Of Coding Gene In Hulless Barley

Hulless barley (Hordeum vulgare L. var. nudum Hook. f.) whose name is called the naked barley(2N=14), Belonging to the Hordeum Linn in Poaceae, and is Variant of Cultivated barley. Hulless barley is the Honorific appellation by People in the China’s Qinghai-Tibet Plateau, which rich in Nutritional quality. In recent years, the development of barley processed proposed new requirements to the processing quality.The grain glutenine is one of the important manufacturing propertie of hulless barley, high molecular weight glutenin subunits(HMW-GS) and low molecular weight glutenin subunits(lmw-gs) are crucial factors for gluten forming. Study of HMW-GS and LMW-GS of hulless barley, would had Significance for improving the market value, efficiently using for the flour and breeding, and provide a scientific foundation for molecular mechanisms of barley quality.In the study,905hulless barley varieties were selected in the barley main producing areas, by using the SDS-PAGE, get the database, and analysis the diversity, the H1genes of HMW-GS were also cloned. The result showed:1. Separation and diversity of HMW-GSTested materials were isolated4HMW subunit (H1, H2, H3, H4), and most of the material was isolated only one band, double subunits only exist in a few species.5kinds of HMW-GS type were isolated:H1、H2、H3、H4、H2+H4, which of the total number Represently:0.55%、95.58%、0.11%、3.21%、0.55%.2. Separation and diversity of LMW-GSLMW-GS have more bands, B-LMW-GS contain2-4bands each species, and C-LMW-GS contain4-7bands. the results of cluster analysis and genetic distance calculation show that there’s no relationship between distribution of LMW-GS and different region, Tibet as the main producing areas and markets in many provinces, it had a most diversity of LMW-GS. 3.Cloning of HMW-GS H1By cloned the H1in hulless barley and analyzed the sequence, we found the mainly differences between H1, H2, H3, H4and D hordein were missing numbers of repeated sequences in the coding region, the performance of it was difference in the number of QTTVSPHQGQ. Meanwhile, we found HMW-GS and D hordein had high structural similarity after contrasted H1, H2, H3, H4and D hordein, and this conclusion is also match the point of HMW-GS had a certain connection with gliadin.