Isolation Of Cellulase Production, Mutagenic And Biological Characteristics

This study aims to separate and identify bacterium producing cellulose enzymefrom Luoyang, Shangqiu, Zhoukou, Luohe et al in eight cities in Henan province.The58samples are derived from the soil of the rice straw hay below5-10cm. Thecellulose enzyme production strain are cultured in the Congo red flat for largertransparent circle and Sodium carboxymethylcellulose as the substrate of the selectivemedium circle.3,5-dinitrosalicylic acid method is used to determine the celluloseenzyme activity. For the screening of strains colony characteristics, microscopicmorphology, physiological and biochemical tests and16SS rDNA sequence areconcerned to identify its species.Biological Characteristics of filter out the high-yield strains of cellulase activity.Strains by UV induced mutation and nitrite with3,5-dinitrosalicylic acid method todetermine the cellulose enzyme activity.Bacteria liquid are disposed and by5%concentration of vaccination in strawfermentation culture concentration, respectively, in72h,96h and120h. straindegradation ability are determined through measuring Microbial populations, crudefiber after the degradation, crude protein contentare and therefore provide a theoreticalbasis for cellulase producing strains in straw degradation.The results showed that the isolated from different samples of the communist partyhas10strains of enzyme activity of higher cellulase enzyme activity in all42strainsthrough the sieve screen.By the Determination of filter paper enzyme activity andsodium carboxymethylcellulose enzyme activity, B.L Y02are found to have bothability, respectively0.5351U/m L and0.3654U/m L.The microscope tablepreliminarily shows the strain was the short rod, in spore form, and accord with bacillusstrains by physiological,biochemical identification and16S rDNA molecular.The biological characteristic of the strain B.L Y02revealed that the optimumgrowth temperature, growth time,pH is37℃,24h,5.0respectively. enzyme activity After mutagenic ultraviolet mutagenesis and nitrite were0.7187U/m L and0.7437U/m L, increased by34.31%and39%.Straw fermented in72h,96h,120h material Bacillus and yeast compared with thecontrol group was highly significant (P <0.01), the number of bacteria of the twomaterials have reached the peak after96h of fermentation, fermentation120hthe numberof microorganisms96h difference was not significant (P>0.05). Crude fiber contentmaterial120h degradation rate up to11.65%, crude fiber content of3.9percentagepoints lower than the raw materials; crude protein content in the120h increase the rateup to44.80, the crude protein content than the materials increased by3.49percentagepoints.