Wnt10b,β-catenin, FGF18Expression In Hair Follicle Morphogenesis During Late Embryonic Development In Gansu Superfine Wool Sheep

Hair follicle morphology characteristics and development regularity is not onlyimportant for its biological characteristics, and have a major impact on these traits.Hair follicle morphogenesis depends on a series of regulation of signaling pathways,these signaling molecules mediated interaction between dermal cells and epithelialcells, induced to stimulate orderly proliferation and differentiation of the two cellpopulations, and ultimately the formation of mature hair follicles, Wnt10b/β-catenin,FGF18gene plays a key role in the process of hair follicle.Histological techniques, Real-time PCR and immunohistochemical were used toresearch the changes of skin organizational structure, expression of Wnt10b/β-catenin and FGF18on mRNA and protein level in Gansu Superfine Wool Sheepfetal of different ages, in order to clarify Gansu Superfine Wool Sheep fetal skin hairfollicle tissue morphology changes and Wnt10b/β-catenin, FGF18gene in GansuSuperfine Wool Sheep hair follicles form occurs during the development of themechanism to provide a theoretical basis.The results showed that:In Gansu Superfine Wool Sheep fetus side skin, the full cuticle structure wasshaped at81d, which gradually differentiated into papillary layer and lamina reticular.The full cuticle structure remained a steady state at123~138d. Primary folliclesprecordium of rib firstly occurred at87d. Secondary follicles were branched off fromthe cuticles near which near the primary follicles which are being developed.Gestational age108d follicle hair follicles tip keratin fibers, gestational age111d hairgrow dry the surface of the skin. The development of primary follicles was fastest at102～123d，Gestational age117to120d visible the villus surface piercing. Theformation of the hair follicle base is a tertiary follicle group, gestational age126d S/P to the highest value of9.96.FGF18mRNA relative expression level gradually increased in Gestational age87d~108d, reached the maximum13.67±0.207gestational age108d; the gestationalage111d, the the FGF18mRNA relative expression level decreased to2.017±0.421,the gestational age141d, the the FGF18mRNA relative expression of11.67±0.523.Immunohistochemical studies have shown that FGF18protein expression waspositive in the fetal epidermis, dermis cytoplasm nucleus;102d~108d, FGF18proteinin the cytoplasm of the hair bud cells were strongly positive，expression of negativenucleus.111d to123d, FGF18protein In the hair follicle ORS, IRS and HS cytoplasmand nucleus were strongly positive， the expression of FGF18protein In the the hairmatrix cytoplasm were positive, a small amount of the dermal papilla cytoplasmic the expression of positive.Conclusions：Secondary-derived follicle from the secondary-original follicledifferentiation secondary follicle branch rather than directly from the epidermis InGansu Superfine Wool Sheep, wool quality S/P ratio of the direct reaction.Wnt10b play an important role in hair follicle morphogenesis and differentia-tion.β-catenin in the hair follicles of the fetal skin, the quality of the developmentprocess from the maintenance of epithelialother signal transduction pathway.FGF18in fetal skin formation and skin appendages, played an important role in thedevelopment process.