The Morphology Of Vorticella Campanula And Investigation Of Sessile Ciliate Attached On Eriocheir Sinensis

Sessile peritrich ciliates was a common organisms attached on crustacean,aquatic organisms, inanimate matter in fresh water and seawater, which ingestedbacterium, organic particles and nanoplankton. It’s food didn’t from attachment, butcould severely impaired all kinds of crustacean’s eggs, larvas and adults, which was aharmful group with wide concern. Shanghai chongming was famous for Eriocheirsinensis offsprings, and In recent years, adult crab has a larger development. This studysamples taken from the region. Eriocheir sinensis was one of important economicspecies of freshwater aquaculture in our country. Sessile peritrich ciliates was one ofpathogen, so was widely concerned. Part kinds of sessile peritrich ciliates attached onthe surface of Eriocheir sinensis with mass propagation and interfere with themovement, foraging and remove shell. Especially, attached on gills that lead to death.The smaller the crab, affected and the greater the damage. Although relatively smalldamage to the a crab, but a large number of parasitic effects its commodity value. So itis necessary to research pathogen morphology, life history and epidemiology of sessileperitrich ciliates disease, which was oder to provided basic research materials withsessile peritrich ciliates disease pathogen investigation, detection, identification andcontrol in Eriocheir sinensis aquaculture process.The morphological characteristics of Vorticella campanula attached onEriocheir sinensis were redescribed by the observations of living bodies and protargoland silver nitrate impregnated bodies. Some new morphological supp lementedcharacteristics were that: One contractile vacuole was7.5-8.7μm in diameter. Freedommovements of some yellow, green, brown and black granules were within theprotoplasm. Food vacuoles were9-15μm in diameter. Stalks were5.1-7.6μm in width. Myonemes were2.7-3.2μm in width, on which some black granules in the diameter of0.5-0.7μm were densely distributed. The clear striaes were on the outside of pellicleand the granulars were in the inside of pellicle. P1and P3synchronous combine andend in the end of the mouth vestibular, form the membrane with five kineties. P2wasvery short and end in the intersection of P1and P3. The outer two kineties were longerthan the inner kinety of P3. The three kineties of P2were equal in length and end ofjunction of P1and P2. The outer two kineties were longer than the inner kinety of P1.The argyrophilic grains were obvious and densely and uneven distributed. Phylogeneticanalysis indicates that V. campanula was located on the base of Vorticella, and in thebetween C. polypinum and others Vorticella. Vorticella and C. polypinum close geneticrelationship. V. campanula was more original than other Vorticellas and was dividedfrom Vorticella at the earliest stage.Live tracking that V. campanula’s asexual reproduction way was diversity,including five ways:1) After V. campanula binary fission, formed two body status. Oneof the body grew pillars telotroch, and out of the mother. Telotroch to find suitablehabitat to come down, and then develop into normal monomer.2) V. campanula inaboral ciliary wreath to generate a ciliary wreath, then development a pillars telotrochand out of the mother. Telotroch to find suitable habitat to come down, and thendevelop into normal monomer.3) V. campanula in aboral ciliary wreath to generate aciliary wreath, then out of the mother. It was developed a pillars telotroch duringswimming. Telotroch to find suitable habitat to come down, and then develop intonormal monomer.4) V. campanula out of the mother, it was generated a ciliary wreathin aboral ciliary wreath during swimming. Telotroch to find suitable habitat to comedown, and then develop into normal monomer.5) V. campanula out of the mother, thendeveloped a telotroch during swimming, and generated a stalk on scopula.According to investgated to Vorticella, Zoothamnium and Epistylis, found theattachment parts showed a certain bias, with shells for most, appendages second, chelarelatively minimal. The attachment parts in the majority quantity with Vorticella.Vorticella attachment quantity showed relatively stable in each area each month, andZoothamnium attachment relatively minimal in each area each month. The biomass peakappeared in August. The quantity in June to August has a tendency to increasedgradually, and began to decline in September. According to detected the water quality ofEriocheir sinensis aquaculture pond, show that the water temperature rise with the month, the maximum from June to September, and began to decline in October. The pHis relatively stable, September and October are relatively low. Dissolved oxygen in Julyand August are relatively high, September began to decline, the lowest in OctoberAmmonia nitrogen is relatively high in July, every other month is relatively stable.Nitrite nitrogen relatively high in July, every other month is relatively low. Activephosphorus are relatively high in July, every other month is relatively stable. Totalorganic carbon in July and August are relatively high, the inorganic carbon is relativelystable, total carbon relatively high in August. Water temperature, pH, dissolved oxygen,and total organic carbon and total carbon to attachment quantity has a significant impact,within a certain range with the groups attachment quantity has a certain positivecorrelation.