The Studies On The Nutrients、Microbial Composition And Dynamic Change Of The Corn Straw Knead Wire Microbial Silage

Objective: As the development of grain-saving animal husbandry, promoting theeffective use of straw, Compared with knead wire microbial silage and traditionalsilage analysis of corn straw, From the conventional feeds nutrients、microorganismsand dynamic three aspects discusses its merits.Methods:（1） The conventional nutrients of knead wire microbial silage andtraditional silage was determined, The determination of water was used directed drying,and crude protein was determined by the Kjeldahl method, Crude fiber was used byacid alkali washing method, Crude fat was used by Soxhlet extraction, Crude ash wasused by weight loss of high temperature burning, Calcium was determined bypotassium permanganate titration, Phosphorus was determined by the molybdenumyellow colorimetry and Nitrogen free extract was used by indirect calculation method.（2）The microbial species and quantity of knead wire microbial silage andtraditional silage was determined, Lactic acid bacteria was used by MRS medium,Yeasts and molds was used by Bengal medium, Bacteria was used by nutrient agarmedium, Actinomycetes was used by G1synthetic medium for culture, and finallycounts.（3） The corn straw longitudinal drawing、 rub while adding straw biologicalmaterial storage modulators in bottles was made by knead wire microbial，andtransverse cutting in bottles was made by traditional silage. The materials of kneadwire microbial and traditional silage were got bottles the fermentation processingsamples at1,2,3,5,6,7,8,9,10,11,12,15,16,18,20,22,26,28,30and35daysduring the fermentation processing were collected. The samples With0.85%sterilesaline were soaked, and then the main microorganism （lactic acid bacteria, yeasts andmolds） were cultured with selective culture medium. Results:（1） The content of nitrogen free extract、crude fiber、neutral andacid detergent fiber in knead wire microbial silage compared with traditional silagewere reduced by5.19%、2.34%、1.44%、1.64%,while crude fat and crude proteincontent increased by5.4%and1.14%, water、crude ash、calcium and phosphoruslevels the difference was not significant.（2） The number of lactic acid bacteria in corn straw knead wire microbial silagewas more than traditional silage by1.54×10¹2cfu/g and the number of yeasts、fungi、actinomycetes and bacteria were less than traditional silage by2.21×10⁷cfu/g、1.23×10⁶cfu/g、4.12×10⁶cfu/g and3.11×10⁴cfu/g.（3）①pH of knead wire micro storage reduced to4at the fifth day and then stayedat about3.8, the overall level was lower than that of traditional silage.â‘¡The numberof lactic acid bacteria of knead wire micro storage increased rapidly and reached to thepeak of1011orders of magnitude at the5th or6th day then reduced slowly and the twopeak appeared at11th days and stabled state at about20th.Lactic acid bacteria in atraditional silage appeared the two lifting changes, the peak reached10¹⁰magnitudeand stabled state at about22th.â‘¢The number of yeast of knead wire micro storagehave fluctuations at the initial stage of fermentation, appeared the peak of106orders ofmagnitude after the number decreases with time and disappeared after the26th day.The number of yeast of traditional silage rapid increased at the within24hours offermentation then reduced slowly, And finally disappeared after the30th day.â‘£Thenumber of mold of knead wire microbial and traditional silage reduced sharplyand no mold was found after3th day.Conclusions: Compared with knead wire microbial silage and traditional silageanalysis of conventional feeds nutrients、microbial pecies and quantity and majorchange of the number of microorganisms，the knead wire microbial silage is morebetter and worthy to be popularized.