The Effects Of Banana Leaf On Various Indexes And Microbial Polymorphism In Rumen Fermentation Of Black Goat

This experiment use Black goats in Guangxi as experimental animal and was designed by single factor, using banana leaf instead of elephant grass, dividing into6levels:without banana leaf (Control group),20%(Diet group1),40%(Diet group2),60%(Diet group3),80%(Diet group4),100%(Diet group5). All of groups were cultured by gas production system in vitro for72h to evaluate the nutritional value of banana leaf as feed so as to provide a theoretical basis for the development and utilizati-on of banana stalks as goat fodder.This thesis includes two parts, gas production metho-d in vitro and the analysis of Microbial Polymorphism in rumen fluid. The main results are as follow:1. The cellulase and xylanase activities of Diet group1were both the highest, separately as0.0135umol/ml min-1and0.0312umol/ml min-1; while diet group2were the lowest, separately as0.0043umol/ml min-1and0.004umol/ml min-1. There was no significant difference on enzyme activity among diet group2,3,4, and5;2. The gas production showed the increasing trend in various diet groups, whose rate increased before36h but decreased after36h. Diet group3had the most gas product ion as40.8ml; While diet group5had the least as31.1ml;3. pH of fermentation substrate in various diet groups increased first and then decreased. pH of diet group3was the highest as6.54, close to diet group4; while the control group was the lowest as6.36;4. The degradation of dry matter decreased first and then increased. The degradation rate of dry matter control group was the highest as10.85%; While diet group3was the lowest as9.82%;5.The amount of protozoa showed gradual down -ward trend, with the highest in control group as8.51×104ml-1but the lowest in diet group5as3.82×104ml-1;6. The Bacterial nitrogen content increased first and then decreased. The Bacterial nitrogen content of diet group3was the highest as0.785mg/ml, while the control group was the lowest as0.675mg/ml;7. With the increas ing quantity of banana leaf added into, Polymorphism of microorganisms decreased gradually.Consideri-ng all factors, the amount of banana leaf added into goat diet is60%.