| This study used endangered plant-Handeliodendron bodinieri seeds as experimental material, preparation5different moisture content with its seeds by silica gel drying method except the initial moisture content, and stored them at room temperature and4℃. Regular dested two kinds of storage conditions water content of seeds in different periods of relative electrical conductivity, soluble sugar, soluble protein, SOD, POD, CAT activity and MDA content in the dynamic changes of physiological and biochemical. Processed germination experiment in sowing date (March), tested the seeds vigor through indicators such as germination proportion, germination potential, germination index, vigor index and so on. Explored its seeds ultra-dry storage characteristics and physiological and biochemical mechanism, screened the best method of treatment of Handeliodendron bodinieri seeds ultra-dry storage. The results showed that:1. Different moisture content seeds under the room temperature and4℃stored Od,30d,60d,90d and160d, each physiological and biochemical indicator was generated dynamically change. As the moisture content decreases, the value of relative electrical conductivity showed a slowly increasing trend, soluble sugar and soluble protein content, SOD activity, POD activity, CAT activity and MDA content showed ups and downs in different period, the soluble sugar and soluble protein content, POD activity were smaller than before storage, and SOD activity, CAT activity and MDA content were increased compared with before storage. 2. Under the storage condition of room temperature, the moisture content ranged from7.72%to3.14%, the Handeliodendron bodinieri seeds of soluble sugar and soluble protein content and SOD activity, POD activity, CAT activity were relatively high, and MDA content was relatively low, indicating that within the moisture content range was beneficial to Handeliodendron bodinieri seeds storage; The principal component analysis and fuzzy comprehensive evaluation showed that, the seeds comprehensive score was highest under the moisture content of7.72%, followed by4.77%and3.97%moisture content, Therefore, the Handeliodendron bodinieri seeds of ultra-dry storage, the optimum moisture content ranged from7.72%to3.97%; The moisture content of4.77%Handeliodendron bodinieri seeds was only smaller0.001than the moisture content of7.72%in comprehensive score, and in the entire storage period, the water content of4.77%Handeliodendron bodinieri seeds relative conductivity and MDA accumulation were less than the moisture content of7.72%, its membrane integrity higher and less accumulation of harmful substances, Therefore, the optimum moisture content was4.77%Handeliodendron bodinieri seeds under the storage condition of room temperature.3. Under the storage condition at4℃, with different moisture contents between each indicator changed small difference; The fuzzy comprehensive evaluation showed that, the seeds comprehensive score was highest under the moisture content of3.97%, followed by the control seeds, and the seeds of the moisture contents of3.14%and7.72%score the third and fourth. Therefore, the Handeliodendron bodinieri seeds of ultra-dry storage at4℃, the optimum moisture content was3.97%.4. Ultra-dry seeds stored in two different storage conditions to sowing, the seeds had a high germination rate, but the two kinds of storage conditions variation in seeds vigor under different moisture contents; At room temperature, as the seeds moisture content decrease, germination proportion, germination potential, germination index and vigor index showed a downward trend, the moisture content of7.72%seeds germination proportion and germination potential were93.182%, germination index was4.950, vigor index was2.718; Followed by the moisture content of4.77%, germination proportion and germination potential were92.500%, germination index was4.525, vigor index was1.966.5. Under the condition of4℃, the moisture content of7.72%, the seeds of germination proportion, germination potential, germination index and vigor index respectively were95.455%,29.545%,3.357,1.379; The moisture content of3.97%seeds germination proportion, germination potential, germination index and vigor index were81.818%,27.273%,2.164,0.834. Comparison of seeds vigor index under different temperature, not ultra-dry seeds vigor was significantly higher than that at room temperature; The moisture content of7.72%under the room temperature and4℃, the seeds vigor were higher than other moisture content, but no significant difference between the two temperature; The seeds vigor under room temperature were higher than at4℃with the moisture content ranged from4.77%to2.54%.6. Considered from the perspective of energy saving and environmental protection, convenient, the best storage method of Handeliodendron bodinieri seeds was silica gel dehydration to a moisture content of4.77%, Sealed storage at room temperature, the membrane system of seeds remains intact and had high vigor. |
