Effects Of Mycorrhizal On Physiological Characteristics And Secondary Metabolites Of Amur Cork And Japanese Yew Seedlings

Amur cork (Phellodendron amurense) is the broad-leaved tree in the angiosperm Rutaceae, and Japanese yew (Taxus cuspidata) is the coniferous tree in the gymnosperms Taxaceae. Both of them contain secondary metabolites having important medicinal value, which are rare medicinal plant resources in Northeast of China and also the national Endangered Species. Mycorrhizal can promote the growth of plants, and affects processes of secondary metabolism. In this paper, it takes seedlings of2-years Amur cork and4-years Japanese yew as experimental subjects,to study the influence of arbuscular mycorrhizal fungi (AMF) to growth of seedlings, Physiological Characteristics and content of secondary metabolites. Results are as follows:The Infection rates of Amur cork seedling which was Inoculated Glomus Mosseae (Gm group) and Glomus versiforme (Gv group) were77.5%and67.1%, infection rate of the control group (CK) was11.2%.Amur cork seedling inoculated with AMF, underground biomass, aboveground biomass, whole plant biomass and root shoot ratio were higher than that of CK, but there was no significant difference. Amur cork seedling with AMF produced more underground biomass than aboveground one, Gm had larger influence on Amur cork seedling than Gv.The net photosynthetic rate (Pn), maximum photosynthetic rate (Pmax), light saturation point (LSP), intercellular CO2concentration (Ci) and stomatal conductance (Gs) of Amur cork seedling for Gm group and Gv group were higher than CK, but the light compensation point (LCP) was lower than CK. The Ci of the Gm and Gv groups had no more difference, but just little higher than CK. The Gs of Amur cork seedling inoculated with AMF was generally higher than CK, but the Gm group was more higher. Each set of parameters was not significant, but AMF can significantly improve photosynthetic efficiency of Amur cork seedling.The maximum fluorescence (Fm), PS II photochemical efficiency (Fv/Fm), PS II potential activity (Fv/Fo), photochemical quenching (qP) and photosynthetic electron transport rate (ETR) of Amur cork seedling for Gm and Gv groups were higher than CK. Basis fluorescence (F0) and non-photochemical quenching (qN) of Gm and Gv groups were lower than CK, and there was no significant difference.In addition to the berberine in leaves of Amur cork seedling, the content of berberine, palmatine and jateorhizine in the group inoculated AMF was varying degree higher than CK. Gv was better than Gm on increasing content of berberine, palmatine and jateorhizine in root.Gv was better than Gm on increasing the content of berberine and palmatine in stem. Gm was better than Gv on increasing the content of palmatine in leaves and jatrorrhizine in stem. Gv had very significant affection on the content of berberine, jatrorrhizine for Amur cork seedling, Gm had significant affection on the content of palmatine.Infection rates of six group of Japanese yew seedlings were between5%and10%. Those light response curve was similar, but Pn of seedling Gv group was higher than other two groups, Pn of cutting Gv group was the lowest. For the seedlings inoculated with AMF, Fo, Fm, Fv/Fm, Fv/Fo, qN and qP were higher than CK. For the cuttings inoculated with AMF, Fv/Fm and Fv/Fo were higher than CK. For the cuttings inoculated with AMF, Fv/Fm and Fv/Fo were both higer than CK. The Fm, Fv/Fm, Fv/Fo and qN of seedlings for Cv and CK were higher than cuttings. Japanese yew inoculated with AMF and the control group, the seedling group and the cutting group had no significant difference.