Preparation And Pharmacodynamics Study On Compound Policresulen Gel

Objectives: to prepare compound gel agent taking policresulen as main medicine fortreating female endometritis, and research deeply and systematically into compoundpolicresulen gel from the physical and chemical properties, stability, security, thebacteriostatic effect in vitro and clinical efficacy, to determine whether this drug can be a newtreatment of endometritis with efficiency and safety, therefore, to provide effective theorybasis for the development in veterinary clinical applications of compound policresulen gel.Methods:(1) To select the best drug formula for compound policresulen gel preparation,by the shape observation, the pH test, the main drug identification and content determinationas the quality evaluation of the preparation.(2) To examine the stability of compoundpolicresulen gel by accelerating experiment, room temperature sample observing test andcentrifugal experiment.(3) To test the safety of compound policresulen gel by acute toxicitytest in mice, maximum stomach tolerance test in mice and eye mucous membrane irritationtest in rabbit.(4) The in vitro pharmacodynamics research was done by in vitro bacteriostasisexperiment to determine the minimum bacteriostatic concentration of compound policresulengel against E. coli and S. aureus.(5) To study the clinical pharmacodynamics of compoundpolicresulen gel, its clinical effect of treatment to sow endometritis was compared to cordatehouttuynia perfusion liquid, also the degree of illness, the dosage and dosing frequency wereinvestigated.Results:(1) The survey of the preparation and the physical and chemical properties ofcompound policresulen gel. The compound policresulen gel was pale yellow, uniform andtransparent viscous gel, its formulation and preparation technology is simple, its pH values at4.5-5.0range, its quality standards comply with the provisions of the2010edition of"Chinese pharmacopoeia".(2) The stability test of compound policresulen gel. Thecompound gel agent are uniform transparent without delamination, the main medicinepolicresulen content changes little, the pH is stable as vaginal gels with a minor drop,indicating that its property is stable.(3) Safety test of compound policresulen gel. The druglavage maximum tolerance factor in mice is270times, and there is no obvious stimulation of eye mucous membrane in rabbit, it can be concluded that compound policresulen gel is safeand complies with the provisions of the mucosa drug preparations.(4) The in vitrobacteriostasis experiment of compound policresulen gel. Compound policresulen gel hasinhibitory effects on both E. coli and S. aureus, the minimum bacteriostatic concentration wasrespectively the0.25and0.5times of drug dilution.(5) The clinical curative effect ofcompound policresulen gel to sow endometritis. The cure rate and total effective rate ofcompound policresulen gel to sow endometritis were78.26%and95.65%, respectively, andthey are significant compared with the control group of cordate houttuynia, indicating thatthis drug is effective in treating dam endometritis.Conclusions: The compound policresulen gel is light yellow,transparent and evenly, withstable physical and chemical properties, low toxicity, low irritation and high security.Compound policresulen gel has certain antibacterial activity against both E. coli and S. aureus,the clinical trials of sow endometritis is effective.