Effect Of Phytase And Xylanase On The Performance, Egg Quality, Blood Parameters And Nutrient Metabolic Rate Of Laying Hens

Five hundred and four40-week-old laying hens were selected and randomly allocated into7treatments with6replicates of12birds. The frist group was positive group, fed with normal diet; the second group was negative group, fed with low calcium, low phosphorus, low-energy diet. The thrid to five groups were supplemented with xylanase and phytase, which adding300u/kg phytase and50g/t,250g/t,350g/t (xylanase one, powdery);50g/t,100g/t (xylanase two, crystals).The experiment was conducted to study the effects of the different forms and different levels of xylanase when adding300u/kg phytase in low calcium, low phosphorus, low-energy diets to investigate effect the performance, egg quality(eggshell strength, eggshell thickness, albumen height, haugh unit), blood parameters (calcium, phosphorus,T3,T4),nutrient utilization(energy utilization, calcium utilization,phosphorus utilization),to determine optimal xylanase and phytase supplementation in laying hens.Experiment1Effect of xylanase and phytase on performance, egg quality and blood parameters, egg production rate of the test three group and the test five group was significantly higher than the negative control group (P<0.05).feed-egg ratio of the test three group, the four group and the five group was significantly lower than the negative control group (P<0.05). egg shape index and eggshell strength were no significant impact (P>0.05) in six weeks, eggshell thickness of the test three group and the test five group was significantly higher than the negative control group (P<0.05), and was no significant impact with the positive control group in six weeks (.P>0.05). albumen height of the test five group was significantly higher than the negative control group in six weeks (P<0.05).egg shape index,eggshell strength,albumen height were no significant impact in groups in twelve weeks (P>0,05). eggshell thickness of the test three group was significantly higher than other test groups (P<0.05).the test groups were significant impact with the positive control group (P>0.05), the positive control group and the test groups were significantly higher than the negative control group (P<0.05)Experiment2Effect of xylanase and phytase on nutrient utilization, the positive control group was the the most highest in AME,AME of the test three group was no significant impact with the positive control group (P>0.05); other groups were no significant impact with the negative group (P>0.05).AME of the test three group was significantly higher than other groups (P<0.05).calcium utilizationof the test three group was no significant impact with the positive control group (P>0.05); other groups were no significant impact with the negative group (P>0.05).AME of the test three group and the positive control group were significantly higher than other groups (P<0.05).phosphorus utilization of the test three group and the test one group was no significant impact with the positive control group (P>0.05),the test three group and the test one group were no significant impact (P>0.05)The tests showed that:adding xylanase and phytase in low calcium, low phosphorus, low-energy diets can improve egg production rate, feed-egg ratio,eggshell thickness, energy utilization, calcium and phosphorus utilization. The optimal supplementation was350g/t (xylanase one, powdery);50g/t (xylanase two, crystals) when adding phytase300u/kg.