The Expression Of4Lipid-metabolic Regulating Genes And Their Correlation With The Adipose Biology In Xinjiang Brown Cattle

The genetic background of Xinjiang brown cattle is very complicated. The mechanismbetween complex genetic background, body fat metabolic is closely related to the causes of meatquality, physiology and biochemistry and molecular biology is unclear. This paper researched onthe correlation between the tissue specific expression of gene correlated with lipid metabolismand adipocyte biology. it will lay the foundation to clarify the body fat metabolic regulationmechanism of Xinjiang Brown cattle.This paper applies the research method：1. The test selected20adult health Xinjiang browncattle bulls to slaughter, fasting for12hours after. the subcutaneous fat, perirenal fat,intermuasular fat and muscle samples were taken, The different parts of the adipose tissue leptingene (lep), fatty acid synthase gene (fas), lipoprotein lipase gene (lpl), and obesity-associated (fto)gene expression was measured by RT-PCR method.2. The tissue sections of subcutaneous fat,perirenal fat, intermuasular fat and pericardial fat samples were made by frozen sections. thenumber of fat cells in the area and per unit area of the fat cells were calculated and analysis thecorrelation with it and the correlation of four lipid metabolism genes expression and fat cellmorphology by the Motic microscopic image processing system.3.The10kinds of lipidmetabolism related hormones and11kinds of blood lipid metabolism enzymes were measured andcorrelation analysis between the four lipid metabolism regulation of gene expression by enzyme-linked immunosorbent (ELISA) and radioimmunoassay (RIA) method.The main results are as follows: The four parts adipose tissue of Xinjiang brown cattle, inaddition to fas gene expression differences are not significant;the gene expression of lep、lpl inthe intermuasular fat and pericardial fat is stronger than other parts;fto expression is the strongestin pericardial fat.The same position adipose tissue expression of each gene showed differences: the geneexpression of lep was significantly higher than other genes in intermuasular fat, the geneexpression fas amount was significantly higher than other genes in the subcutaneous fat(P<0.05),the lpl, fto gene expression was significantly (P<0.05) higher than other genes in theperirenal fat, the fto gene expression was significantly higher than other genes in the pericardial fat.The area and number of fat cells there are tissue-specific, various parts of the fat cell sizeorder: perirenal>subcutaneous, pericardial and intermuasular. Per unit area of cell numbersequence: the subcutaneous and intermuasular>pericardial and perirenal. The lep the expression ofintermuasular fat was negatively correlated with the parts of the area of the fat cells (r=-0.3656;P<0.05), which with lpl gene (r=-0.51; P=0.0581) expression levels have tend to becorrelated; the lep, lpl （r=-0.4133, r=-0.47； P<0.05） the expression of perirenal fat wasnegatively correlated with the parts of the fat cell area.the cell area of perirenal fat wassignificantly correlated with four lipid metabolism regulation of gene expression(P>0.05). the cellnumber of perirenal fat was significantly correlated with four lipid metabolism regulation of geneexpression(P>0.05). the gene expression of lep and blood LPL(r=0.88P<0.01,0.92;P<0.05) andFAS (r=0.87,0.90;P<0.01) concentration showed a strong positive correlation in intermuasular andpericardial.the gene expression of lpl and blood LPL(r=0.82P<0.01,0.63; P<0.05) and FAS(r=0.80,0.61; P<0.05) concentration showed a strong positive correlation in intermuasular andsubcutaneous.the gene expression of lep and blood LEP(r=0.87,0.92, P<0.01; r=0.52, P<0.05)concentration showed a strong positive correlation in intermuasular,perirenal and pericardial.thegene expression of lpl and blood LEP(r=0.82,0.62; P<0.01) concentration showed a strongpositive correlation in intermuasular,perirenal and pericardial. the gene expression of fto and bloodLEP(r=-0.78; P<0.01) concentration showed a strong negative correlation in intermuasular.By giving an analysis to the result of the study, the conclusions are summed as follows:Thefour fat metabolism regulation of gene expression has tissue specificity of Xinjiang brown cattle.The fat cell area,number has tissue specificity in different parts of Xinjiang brown cattle.The results suggest that may be related to the number of preadipocytes, differentiation, bloodsupply and hormonal regulation. Four lipid metabolism regulate of gene expression be related tocell morphology. in the development of latter part, It tip that fat deposition were mainly fatdroplets filling cells, not obvious proliferation. Four lipid metabolism regulation of gene expression associated with11and10fatmetabolism correlation of enzymes, hormones analysis showed that LPL blood, the lepconcentration may regulate adipose tissue, expression; gene of FAS, LPL. Blood LEP, FAS, LPL,FTO adjustment affects the body fat metabolism gene expression.The study provides physiological biochemistry and molecular biology basis for theregulation of lipid metabolism mechanism of Xinjiang brown cattle, witch laid the foundation formolecular markers for meat quality breeding. explore its mechanism of regulation of lipidmetabolism.