Establishment Of Canine Partial Hepatectomy Model And Its Repairment And Regeneration In Post Operation

Objective: Liver injury caused by external forces in pets is one of the common diseases in clinics; theoperation is one of the effective tools to safe the life. However poor prognosis occurs sometimes. Thecanine partial hepatectomy was applied in this study to establish a dog model combined withhepatoprotective therapeutics to evaluate the effectiveness of liver recovery and regeneration thus laying afoundation for good treatment. Materials and Methods: Eight2-year-old healthy dogs were randomlydivided into two groups of each four dogs, i.e., anti-inflammatory group (AI) and anti-inflammatory+hepatoprotective treatment group (AI+HPT). In AI group anti-inflammatory drugs were used, and inAI+HPT group anti-inflammatory drugs combined with hepatoprotective drugs were used after partialhepatectomy. About1/5of left lateral lobe of liver was surgically cut in each dog, with sampling of livertissues as control for histo-pathological and molecular analysis. The body temperature, respiration, pulseand blood physiological and biochemical parameters were monitored at day1,3,5,13,21and29after thesurgery. The anti inflammatory and infection drugs were treated between day1and3after surgery in AIgroup, whereas in AI+HPT group, the additional hepatoprotective drugs were used with anti inflammatoryand infection ones. The hepatoprotective drugs including vitamin C, some medicinal herbs such as Jukeganand glycyrrhizin amine compound were treated for10days after operation. All animal were sacrificed in76days after operation, the liver tissues were sampled for patho-histological and hepatocyte proliferationanalysis. Results: the number of red blood cell was significantly decreased in first two days after operationin both AI and AI+HPT groups (P<0.05) and thereafter reached to the normal level if compared with thatbefore operation. The number of white cell was significantly increased from day3to day5after operationin both AI and AI+HPT groups (P<0.05) and thereafter dropped to the normal level of the pre-operation.Additionally, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were increasedsignificantly at day1after operation then decreased to the normal level as that before the operation. Thatthe number of red blood cell was reached to the normal level was better in AI+HPT group than in AI group.All the blood physiological biochemical parameters measured were recovered to the normal level as that inpre-operation between day21and29after operation. The Path-histological changes of hepatocyte swelling,necrosis and nucleus offset and karyolysis with hepatic vascular wall thickening and vascular stenosis, andconnective tissue proliferation in the liver injury by hepatectomy were observed in AI group but onlyhepatocyte swelling and nucleus offset were observed in AI+HPT group. The hepatic proliferation factorsas PCNA, p-ERK, Cyclin A, Cyclin D1and CyclinE were expressed significantly higher during thepost-operative period than pre-operation showing an obvious repairment and regeneration afterhepatectomy. Conclusion: it is concluded that the canine partial hepatectomy model was established withminimal negative impact on the physiological and biochemical functions in the liver. This study lays apath-physiologically useful foundation for the clinical treatment of liver injury caused by external forces.